Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

PD-L1 (E1L3N®) XP® Rabbit mAb #13684

B7-H1   B7H1   CD274   PD1   PDCD1 Ligand 1   Programmed cell death 1 ligand 1  

No. Size Price
13684S 100 µl ( 10 western blots ) ¥7,500.00 现货查询 购买询价
13684T 20 µl ( 2 western blots ) ¥2,000.00 现货查询 购买询价
13684 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 40-50 Rabbit IgG
IP 1:50
IHC-P 1:200
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

PD-L1 (E1L3N) XP® Rabbit mAb recognizes endogenous levels of total PD-L1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, IFN-γ treated (100 ng/mL, 48 hr; +) or untreated (-), using PD-L1 (E1L3N™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from KARPAS-299, SUP-M2, and PC-3 cells using PD-L1 (E1L3N™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated SUP-M2 cells using PD-L1 (E1L3N™) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Karpas-299 (left) or PC-3 (right) cell pellets using PD-L1 (E1L3N™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 (E1L3N™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human placenta using PD-L1 (E1L3N™) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).

  1. Dong, H. et al. (1999) Nat Med 5, 1365-9.
  2. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  3. Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
  4. Dong, H. et al. (2002) Nat Med 8, 793-800.
  5. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
  6. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
  7. Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
  8. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
  9. Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.
  10. Wimberly, H. et al. (2014) Cancer Immunol Res , .

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

E1L3N is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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