Cell Signaling Technology

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ZO-1 (D6L1E) Rabbit mAb #13663

sc-33725   sc-8146  

No. Size Price
13663S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13663 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 220 Rabbit IgG
IP 1:50
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

ZO-1 (D6L1E) Rabbit mAb recognizes endogenous levels of total ZO-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1558 of human ZO-1 protein.

IP

IP

Immunoprecipitation of ZO-1 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ZO-1 (D6L1E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using ZO-1 (D6L1E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ZO-1 (D6L1E) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 (high-expressing, left) and MDA-MB-435 (low-expressing, right) cells, using ZO-1 (D6L1E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
  3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
  4. Umeda, K. et al. (2006) Cell 126, 741-54.
  5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
  6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
  7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
  8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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