Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Stathmin (D1Y5A) Rabbit mAb #13655

sc-15320   sc-7804  

No. Size Price
13655S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13655 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 19 Rabbit IgG
IHC-P 1:2000
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Stathmin (D1Y5A) Rabbit mAb recognizes endogenous levels of total stathmin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro142 of human stathmin protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using Stathmin (D1Y5A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse kidney using Stathmin (D1Y5A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stathmin (D1Y5A) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Stathmin (D1Y5A) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Stathmin (D1Y5A) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node using Stathmin (D1Y5A) Rabbit mAb.

Background

Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC-12 cells (8).

  1. Marklund, U. et al. (1994) Eur J Biochem 225, 53-60.
  2. le Gouvello, S. et al. (1998) J Immunol 161, 1113-22.
  3. Mistry, S.J. and Atweh, G.F. (2001) J Biol Chem 276, 31209-15.
  4. Gavet, O. et al. (1998) J Cell Sci 111 ( Pt 22), 3333-46.
  5. Luo, X.N. et al. (1994) J Biol Chem 269, 10312-8.
  6. Vancompernolle, K. et al. (2000) J Biol Chem 275, 33876-82.
  7. Daub, H. et al. (2001) J Biol Chem 276, 1677-80.
  8. Di Paolo, G. et al. (1996) J Cell Biol 133, 1383-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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