Cell Signaling Technology

Product Pathways - DNA Damage

Camptothecin #13637

arrest   cycle   damage   DNA   p53   RNA   topoisomerase I  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Molecular Weight:

348.4 g/mol

Molecular Characterization

C20H16N2O4

Purity

>98%

Bioactivity

Solubility: Soluble in DMSO up to 10 mg/ml with warming. Very poorly soluble in ethanol and water.

Structure

Structure

Chemical structure of camptothecin.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Camptothecin (6 hr) at the indicated concentrations, using Phospho-p53 (Ser15) Antibody #9284 (upper), p53 (1C12) Mouse mAb #2524 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Directions for Use

Camptothecin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.87 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 1-24 hr.

Background

Camptothecin is a cytotoxic plant alkaloid originally isolated from C. acuminate that inhibits DNA and RNA synthesis in mammalian cells and is an effective anti-tumor agent (1). Research studies indicate that camptothecin inhibits topoisomerase I with an IC50 of 679 nM (2). Camptothecin binds and stabilizes topoisomerase I–DNA cleavage complexes, which leads to DNA strand breaks (1,3,4). The resultant DNA damage can induce cell cycle arrest in many cancer cell lines (5,6). Inactivation of the tumor suppressor protein p53 can increase the cytotoxicity of camptothecin (6).

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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