Product Pathways - DNA Damage
|13637S||10 mg||￥506.00 现货查询||购买询价|
|13637||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Solubility: Soluble in DMSO up to 10 mg/ml with warming. Very poorly soluble in ethanol and water.
Directions for Use
Camptothecin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.87 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 1-24 hr.
Camptothecin is a cytotoxic plant alkaloid originally isolated from C. acuminate that inhibits DNA and RNA synthesis in mammalian cells and is an effective anti-tumor agent (1). Research studies indicate that camptothecin inhibits topoisomerase I with an IC50 of 679 nM (2). Camptothecin binds and stabilizes topoisomerase I–DNA cleavage complexes, which leads to DNA strand breaks (1,3,4). The resultant DNA damage can induce cell cycle arrest in many cancer cell lines (5,6). Inactivation of the tumor suppressor protein p53 can increase the cytotoxicity of camptothecin (6).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12611 DMSO (Dimethyl Sulfoxide), Sterile
- 2524 p53 (1C12) Mouse mAb
- 9284 Phospho-p53 (Ser15) Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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