Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

RBL2 (D9T7M) Rabbit mAb #13610

p130   sc-317  

No. Size Price
13610S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
13610 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 130 Rabbit IgG
IP 1:100
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

RBL2 (D9T7M) Rabbit mAb recognizes endogenous levels of total RBL2 protein. This antibody does not cross-react with the Rb or RBL1 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to human RBL2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBL2 (D9T7M) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® RBL2 siRNA I # 14712 (+), using RBL2 (D9T7M) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The RBL2 (D9T7M) Rabbit mAb confirms silencing of RBL2 expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 3T3 cells and either 10 µl of RBL2 (D9T7M) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse CCNE1 promoter primers, mouse CDC2 promoter primers, SimpleChIP® Mouse B-Myb Intron 2 Primers #13707, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


The retinoblastoma (Rb) tumor suppressor family includes the retinoblastoma protein Rb (p105), retinoblastoma-like protein 1 (RBL1, p107), and retinoblastoma-like protein 2 (RBL2, p130). These Rb family proteins are referred to as ‘pocket proteins’ because they contain a conserved binding pocket region that interacts with critical regulatory proteins, including E2F family transcription factors, c-Abl tyrosine kinase, and proteins containing a conserved LXCXE motif (1,2). In quiescent G0 phase cells, active Rb proteins are hypophosphorylated and bind to E2F transcription factors to repress transcription and inhibit cell cycle progression (1,2). Upon growth factor induction of quiescent cells, Rb proteins become hyperphosphorylated and inactivated by G1-phase cyclinD-cdk4/6, G1/S-phase cyclin E-cdk2, and G1/S-phase cyclin A-cdk2 complexes (1,2). Hyperphosphorylation of Rb proteins results in a loss of E2F binding and allows for transcriptional activation and cell cycle progression (1,2). In addition to regulating the cell cycle, Rb proteins regulate chromosome stability, induction, and maintenance of senescence, apoptosis, cellular differentiation, and angiogenesis (3).

Retinoblastoma-like protein 2 (RBL2, p130) is the most predominant and active Rb family member found in quiescent cells. In these cells, RBL2 interacts with E2F4 and E2F5 to recruit the DP, RB-like, E2F, and MuvB protein (DREAM) complex to E2F target genes to repress transcription of multiple genes required for progression into S phase and mitosis (4-6). Hypophosphorylation of RBL2 during cellular senescence is required for maintenance of senescent cells (7,8).

  1. Du, W. and Pogoriler, J. (2006) Oncogene 25, 5190-200.
  2. Giacinti, C. and Giordano, A. (2006) Oncogene 25, 5220-7.
  3. Indovina, P. et al. (2013) J Cell Physiol 228, 525-35.
  4. Moberg, K. et al. (1996) Mol Cell Biol 16, 1436-49.
  5. Takahashi, Y. et al. (2000) Genes Dev 14, 804-16.
  6. Smith, E.J. et al. (1996) Mol Cell Biol 16, 6965-76.
  7. Kapić, A. et al. (2006) Cell Death Differ 13, 324-34.
  8. Helmbold, H. et al. (2009) Oncogene 28, 3456-67.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :