Cell Signaling Technology

Product Pathways - Protein Stability

UBLE1A/SAE1 Antibody #13585

AOS-1   AOS1   SAE-1   SAE1   SUMO Activating Enzyme   Sumoylation   UBLE1A  

No. Size Price
13585S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13585 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 40 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,

Specificity / Sensitivity

UBLE1A/SAE1 Antibody recognizes endogenous levels of total UBLE1A/SAE1 protein. This antibody does not cross-react with NAE1 or UBE1 proteins.

UBLE1A/SAE1 抗体识别内源性水平的UBLE1A/SAE1总蛋白。此抗体不与NAE1或UBE1蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys195 of human UBLE1A/SAE1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体经合成与人UBLE1A/SAE1蛋白Lys195邻近的氨基酸残基序列一致的肽段,免疫动物产生。抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBLE1A/SAE1 Antibody.Western blot检测多种细胞提取物,使用的抗体是UBLE1A/SAE1 Antibody。

Specificity

Specificity

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs encoding Myc/DDK-tagged full-length human SAE1 protein (hSAE1-Myc/DDK; +), Myc/DDK-tagged full-length human NAE1 protein (hNAE1-Myc/DDK; +), or Myc/DDK-tagged full-length human UBE1 protein (hUBE1-Myc/DDK; +), using UBLE1A/SAE1 Antibody (upper) or DYKDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞提取物,对照未转染(-)或转染了Myc/DDK标签的全长人源的SAE1蛋白(hSAE1-Myc/DDK; +),Myc/DDK标签的全长人源的NAE1蛋白(hNAE1-Myc/DDK; +), 或Myc/DDK标签的全长人源的UBE1蛋白(hUBE1-Myc/DDK; +),使用的抗体是UBLE1A/SAE1 Antibody (上图)或DYKDDDK Tag Antibody #2368 (下图)。

IP

IP

Immunoprecipitation of UBLE1A/SAE1 from MCF7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or UBLE1A/SAE1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBLE1A/SAE1 Antibody.对MCF7细胞提取物中的UBLE1A/SAE1进行免疫共沉淀,使用抗体是Normal Rabbit IgG #2729 (泳道2)或UBLE1A/SAE1 Antibody (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是UBLE1A/SAE1 Antibody。

Background

The process of SUMO conjugation to target proteins is similar to the molecular chain of events observed with ubiquitin (1). SUMO is conjugated to target proteins through the coordinated action of the cellular SUMO conjugation machinery, which consists of the E1, E2, and E3 enzymes (2). The canonical SUMO E1 activating enzyme is a heterodimer consisting of Ubiquitin-like 1-activating enzyme E1A (UBLE1A, SAE1) and UBLE1B (SAE2, UBA2) subunits. Mature SUMO is activated by E1 in an ATP-dependent reaction that generates adenylated SUMO, which functions as a high-energy intermediate in the formation of a thioester linkage between SUMO and Cys173 of SAE2 (3,4). SUMO is subsequently transferred from SAE2 to the SUMO E2 conjugating enzyme UBE2I (5). Research studies indicate that UBLE1A (SAE1) is a nuclear protein and c-Myc transcriptional target whose expression is required for Myc-driven tumorigenesis (6-8).

SUMO修饰结合于目标蛋白质的过程是类似于泛素化修饰的事件(1)。SUMO是通过由E1,E2和E3酶(2)组成的SUMO修饰系统协同作用并偶联于目标蛋白质。典型的SUMOE1激活酶是由UBLE1A(SAE1) 和UBLE1B (SAE2, UBA2)亚基组成的异源二聚体。成熟SUMO是由激活酶E1通过依赖于ATP的反应,生成腺苷酸化SUMO(SUMO与UBA2的Cys173之间形成硫酯键而形成的一种高能中间体)(3,4)。SUMO然后从UBA2转移到SUMO的E2共价连接酶UBE2I(5)。最近的证据表明,UBLE1A (SAE1)是一种核蛋白,并且是c-Myc的转录靶基因,它的表达是myc导致的肿瘤发生所必需的(6-8)。

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 9959-69.
  3. Desterro, J.M. et al. (1999) J Biol Chem 274, 10618-24.
  4. Gong, L. et al. (1999) FEBS Lett 448, 185-9.
  5. Desterro, J.M. et al. (1997) FEBS Lett 417, 297-300.
  6. Moutty, M.C. et al. (2011) Mol Biol Cell 22, 652-60.
  7. Amente, S. et al. (2012) Am J Cancer Res 2, 330-4.
  8. Kessler, J.D. et al. (2012) Science 335, 348-53.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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