Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

DAX1 (D2F1) Rabbit mAb #13538

No. Size Price
13538S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13538 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit IgG
IP 1:100
IHC-P 1:400
IF-IC 1:3200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

DAX1 (D2F1) recognizes endogenous levels of total DAX1 protein.

DAX1 (D2F1)可以识别内源性的总DAX1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly330 of human DAX1 protein.

此 单克隆抗体由合成肽段免疫动物产生,该肽段与人DAX1蛋白Gly330邻近的氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from U-87 MG, NCI-H460, A431 cells, and human testis using DAX1 (D2F1) Rabbit mAb. A431 extract is negative as expected.

使用DAX1 (D2F1) Rabbit mAb对U-87 MG, NCI-H460, A431 cells和人睾丸组织提取物进行western blot分析。A431如预期所示是阴性的。

IP

IP

Immunoprecipitation of DAX1 from KYSE-450 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DAX1 (D2F1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DAX1 (D2F1) Rabbit mAb.

使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或DAX1 (D2F1) Rabbit mAb (lane 3)对KYSE-450细胞提取物中的DAX1蛋白进行免疫沉淀实验。Lane1是10%上样量。使用DAX1 (D2F1) Rabbit mAb进行western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H460 (positive; left) or A431 (negative; right) cell pellets using DAX1 (D2F1) Rabbit mAb.

使用DAX1 (D2F1) Rabbit mAb对石蜡包埋的NCI-H460 (阳性;左) 或 A431 (阴性; 右)进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human testis using DAX1 (D2F1) Rabbit mAb.

使用DAX1 (D2F1) Rabbit mAb对石蜡包埋的人睾丸组织进行免疫组化分析。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H460 (positive; upper) and A431 (negative; lower) cells, using NR0B1 (D2F1) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用NR0B1 (D2F1) Rabbit mAb (绿色)对NCI-H460 (阳性; 上) 和A431 (阴性;下)进行共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054 (红色)进行分析。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using DAX1 (D2F1) Rabbit mAb.

使用DAX1 (D2F1) Rabbit mAb兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。

Background

DSS-AHC critical region on the X chromosome protein 1 (DAX1) is an orphan nuclear receptor encoded by the nuclear receptor subfamily 0 group B member 1 (NR0B1) gene. DAX1 possesses an atypical DNA binding domain that allows it to form heterodimeric complexes with DNA binding partners and repress transcriptional activity (1,2). During development, DAX1 is important for establishment of the hypothalamic-pituitary-adrenal gonadal axis. The receptor is essential for development of several important hormone-producing organs that determine this axis, including the adrenal glands, pituitary, hypothalamus, and the male and female reproductive organs (3,4). Research studies suggest that DAX1 plays a role in maintenance of pluripotency in embryonic stem cells (5,6). Loss of DAX1 function through deletion or mutation results in adrenal insufficiency and hypogonadotropic hypogonadism (7), while duplication of the NR0B1 gene on the X-chromosome causes dosage-sensitive sex reversal (8).

位于X染色体上的蛋白1(DAX1)的DSS-AHC关键区域是一个孤儿核受体,由孤儿核受体亚家族0 group B member 1 (NR0B1)编码。DAX1含有一个非典型DNA结合结构域与使其能够与DNA结合伴侣形成二聚体抑制转录活性(1,2)。在发育时期,DAX1对建立下丘脑 -垂体 -肾上腺性腺轴是非常重要的。该受体对几个重要的决定该轴发育的激素合成器官至关重要,包括肾上腺,垂体,下丘脑,和雄性和雌性生殖器官(3,4)。研究表明DAX1能维持胚胎干细胞的多潜能性(5,6)。DAX缺失或突变会导致肾上腺功能不全和性腺功能机能减退(7),而X染色体上的NR0B1基因复制会导致剂量敏感的性逆转(8)。

  1. Iyer, A.K. et al. (2006) Mol Endocrinol 20, 2326-42.
  2. Iyer, A.K. and McCabe, E.R. (2004) Mol Genet Metab 83, 60-73.
  3. Niakan, K.K. and McCabe, E.R. (2005) Mol Genet Metab 86, 70-83.
  4. McCabe, E.R. (2007) Mol Cell Endocrinol 265-266, 179-82.
  5. Uranishi, K. et al. (2013) Mol Cell Biol 33, 2056-66.
  6. Wang, Q. and Cooney, A.J. (2013) Adv Exp Med Biol 786, 287-306.
  7. Jadhav, U. et al. (2011) Mol Cell Endocrinol 346, 65-73.
  8. Sanlaville, D. et al. (2004) Am J Med Genet A 128A, 325-30.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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