Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb #13448

No. Size Price
13448S 100 µl ( 10 western blots ) ¥4,180.00 现货查询 购买询价
13448T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
13448 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 69,78 Rabbit IgG
IP 1:100
IF-IC 1:2500

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb recognizes endogenous levels of lamin A/C protein only when phosphorylated at Ser22.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or hydroxyurea-treated (right), using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, treated with either hydroxyurea (4 mM, 20 hr; +) to induce G1/S phase or Paclitaxel #9807 (100 nM, 20 hr; +) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb (upper) and Lamin A/C Antibody #2032 (lower).



Immunoprecipitation of phospho-Lamin A/C (Ser22) from extracts of HeLa cells treated with Paclitaxel #9807, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Lamin A/C (Ser22) (D2B2E) X® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb.


Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).

Phosphorylation of lamin A/C at Ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. The surrounding sequence is a typical MAPK/CDK phosphorylation motif, which implicates a role in the cell cycle and mitosis (7-11).

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  7. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
  8. Molina, H. et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
  9. Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
  10. Nousiainen, M. et al. (2006) Proc Natl Acad Sci U S A 103, 5391-6.
  11. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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