Cell Signaling Technology

Product Pathways - Apoptosis

Lamin B1 (D9V6H) Rabbit mAb #13435

sc-20682   sc-6216   sc-6217  

No. Size Price
13435S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13435 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 68, 45 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,

Specificity / Sensitivity

Lamin B1 (D9V6H) Rabbit mAb recognizes endogenous levels of total lamin B1 protein. This antibody recognizes the 45 kDa lamin B1 carboxy terminal cleavage product produced during apoptosis.

Lamin B1 (D9V6H) Rabbit mAb 兔单抗能够检测内源的lamin B1 总蛋白。该抗体可以识别凋亡时产生的45 kDa lamin B1羧基端切割产物。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys415 of human lamin B1 protein.

该单克隆抗体采用合成的人lamin B1蛋白Lys415周围残基相对应的肽段免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Lamin B1 (D9V6H) Rabbit mAb.

使用Lamin B1 (D9V6H) Rabbit mAb 兔单抗对多种细胞体系和组织提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +), using Lamin B1 (D9V6H) Rabbit mAb.

用未处理(-)或经过Staurosporine #9953 (1 μM, 3 hr; +)处理的Hela细胞提取物,使用Lamin B1 (D9V6H) Rabbit mAb 兔单抗进行western blot分析。

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).

核纤层蛋白Lamin是核膜结构的组分,对维持正常的细胞功能非常重要,例如细胞周期控制、DNA修复和染色质组装(1-3)。Lamins 已细分为A和B型。A型核纤层蛋白由核纤层蛋白A和C组成,来源于选择性剪接。Lamin A/C 能够被剪切成特异的大片段(41-50 kDa)和小片段 (28 kDa) ,这是细胞凋亡的标志(4,5)。B型核纤层蛋白由核纤层蛋白B1和B2组成,由单独的基因编码(6-8)。在细胞凋亡时,核纤层蛋白B1也被半胱天冬酶酶切(9)。研究显示,核纤层蛋白B1基因的复制,与神经功能紊乱并在成年期发病的脑白质病变的发病机制相关(10)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
  7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
  8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
  9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
  10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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