Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

NRMT (D9D6P) Rabbit mAb #13432

No. Size Price
13432S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13432 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 25 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

NRMT (D9D6P) Rabbit mAb recognizes endogenous levels of total NRMT protein.NRMT (D9D6P) Rabbit mAb可以识别内源性NRMT总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu49 of human NRMT protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人NRMT蛋白Leu49附近氨基酸一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NRMT (D9D6P) Rabbit mAb.使用NRMT (D9D6P) Rabbit mAb对多种细胞提取物进行western blot分析。

Background

N-terminal RCC1 methyltransferase (NRMT), formerly known as methyltransferase-like protein 11A (METTL11A), is a member of the methyltransferase 11 family of proteins and is the first α-N-methyltransferase to be discovered in humans (1-3). Amino-terminal methylation of free α-amino groups is a post-translational modification where an initiating Met residue is cleaved and the exposed α–amino group is mono-, di-, or trimethylated by NRMT (4). NRMT methylates proteins containing an amino-terminal Met-X-Pro-Lys motif, where X is an alanine, proline, or serine residue (4). Substrates of NRMT include the Ran guanine nucleotide-exchange factor (RCC1), SET/TAF-1/PHAP-II, retinoblastoma (Rb), and CENP-B (3-6). α-N-methylation of RCC1 is required for efficient binding to chromatin, securing normal bipolar spindle formation and chromosome segregation (3,5). α-N-methylation of CENP-B also appears to regulate CENP-B binding to centromeric DNA (6).N末端RCC1甲基转移酶(NRMT),最初被认为是甲基转移酶样蛋白11A(METTL11A),是甲基转移酶11家族蛋白的一员,也是人体最早被发现的α-N-甲基转移酶(1-3)。free α-amino家族的氨基端甲基转移是一个翻译后修饰过程,其间Met残基被脱离,然后暴露的α-amino家族被NRMT单甲基化,双甲基化或多甲基化(4)。NRMT甲基化含有氨基端Met-X-Pro-Lys基序的蛋白,其中X可以是丙氨酸、脯氨酸、或丝氨酸残基(4)。NRMT的底物包括Ran 鸟嘌呤核苷酸转移因子(RCC1), SET/TAF-1/PHAP-II, 成视网膜细胞瘤(Rb),和 CENP-B (3-6). RCC1的α-N-甲基化对于与染色质的结合,正常双极纺锤体形成和染色质分离都是必须的(3-5)。CENP-B的α-N-甲基化也能调控CENP-B和着丝粒DNA的结合(6)。

  1. Wrighton, K.H. (2010) Nat Rev Mol Cell Biol 11, 604-5.
  2. Webb, K.J. et al. (2010) Biochemistry 49, 5225-35.
  3. Tooley, C.E. et al. (2010) Nature 466, 1125-8.
  4. Petkowski, J.J. et al. (2012) Biochemistry 51, 5942-50.
  5. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
  6. Dai, X. et al. (2013) J Proteome Res 12, 4167-75.

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