Cell Signaling Technology

Product Pathways - Protein Stability

UBE1L2/UBA6 Antibody #13386

E1 Ubiquitin Activating Enzymes   UBA-6   UBA6  

No. Size Price
13386S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
13386 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 117 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Pig,

Specificity / Sensitivity

UBE1L2/UBA6 Antibody recognizes endogenous levels of total UBE1L2/UBA6 protein. This antibody does not cross-react with UBE1/UBA1 or UBE1L/UBA7 proteins.

UBE1L2/UBA6 抗体识别内源性的UBE1L2/UBA6总蛋白。此抗体不与UBE1/UBA1或UBE1L/UBA7发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys810 of human UBE1L2 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBE1L2/UBA6 Antibody.Western blot检测多种细胞提取物,使用的抗体是UBE1L2/UBA6 Antibody.



Immunoprecipitation of UBE1L2/UBA6 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or UBE1L2/UBA6 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE1L2/UBA6 Antibody.对293T细胞提取物中的UBE1L2/UBA6进行免疫共沉淀,使用抗体是Normal Rabbit IgG #2729 (泳道2)或UBE1L2/UBA6 Antibody (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是UBE1L2/UBA6 Antibody。



Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UBE1L2 protein (hUBE1L2-Myc/DDK; +), Myc/DDK-tagged full-length human UBE1 protein (hUBE1-Myc/DDK; +), or Myc/DDK-tagged full-length human UBE1L protein (hUBE1L-Myc/DDK; +), using UBE1L2/UBA6 Antibody (upper) or DYKDDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞提取物,对照未转染(-)或转染了Myc/DDK标签的全长人源的UBE1L2 protein (hUBE1L2-Myc/DDK; +), Myc/DDK标签的全长人源的UBE1 protein (hUBE1-Myc/DDK; +),或Myc/DDK标签的全长人源的UBE1L protein (hUBE1L-Myc/DDK; +),使用的抗体是UBE1L2/UBA6 Antibody (上图)或DYKDDDDK Tag Antibody #2368 (下图)。


Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the ubiquitin-activating enzyme (E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3).

Ubiquitin-activating enzyme E1-like protein 2/Ubiquitin-like modifier-activating enzyme 6 (UBE1L2/UBA6) is ubiquitously expressed in human tissues and functions as an E1 enzyme related to UBE1/UBA1 (40% identity at the protein level). UBE1L2/UBA6 activates both ubiquitin and the ubiquitin-like protein FAT10 through a similar ATP dependent mechanism (4-6). Like other E1 protein family members, UBE1L2/UBA6 contains a conserved ATP-binding adenylation domain and an active site cysteine residue that are critical for enzymatic function (4,5). Research studies have demonstrated that UBE1L2/UBA6 expression is essential during the early stages of embryogenesis in mice (4). Furthermore, loss of neuronal UBE1L2/UBA6 expression promotes significant defects in neuronal structure and function, which contributes to a reduction in body weight and decreased postnatal viability (7).

蛋白泛素化作用需要E1, E2, 和E3泛素-连接酶的协同作用。泛素最先通过具有硫酯的泛素激活酶E1通过ATP-依赖形式活化。活化的泛素然后通过含有巯基的泛素载体蛋白E2转运。最后从E2的泛素转运到含有ε-氨基的赖氨酸残疾靶蛋白,这由泛素连接酶E3介导(1)。

UBE1L2/UBA6在人体各组织中广泛表达并作为UBE1/UBA1(蛋白水平上40%同源)的E1酶发挥作用。UBE1L2/UBA6通过相似的ATP依赖机制激活泛素和类泛素蛋白FAT10(4-6)。像其他E1酶家族成员一样,UBE1L2/UBA6包含一个保守的ATP结合腺苷酰化结构域和一个具有活性的半胱氨酸位点,对酶发挥功能至关重要(4,5)。研究已经表明,UBE1L2 / UBA6表达在小鼠胚胎发育的早期阶段至关重要(4)。此外,神经中UBE1L2 / UBA6的表达缺失导致神经结构和功能显著缺陷,进而致使体重的减少和产后存活率的下降(7)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Chiu, Y.H. et al. (2007) Mol Cell 27, 1014-23.
  5. Pelzer, C. et al. (2007) J Biol Chem 282, 23010-4.
  6. Jin, J. et al. (2007) Nature 447, 1135-8.
  7. Lee, P.C. et al. (2013) Mol Cell 50, 172-84.

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