Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TFIIE-α Antibody #13375

No. Size Price
13375S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
13375 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TFIIE-α Antibody recognizes endogenous levels of total TFIIE-α protein.

TFIIE-α Antibody 兔多抗能够检测内源性TFIIE-α总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly243 of human TFIIE-α protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TFIIE-α Antibody. Western blot方法检测不同细胞系的提取物,使用的抗体为 TFIIE-α Antibody。


Transcription initiation factor IIE subunit alpha (TFIIE-α) is part of TFIIE, a general transcription factor made up of paired α and β subunits. These general transcription factors include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH (1-4). Binding of RNA polymerase II (RNAPII) to promoter sequences as part of the pre-initiation complex (PIC) is facilitated by these general transcription factors. These factors also help in selection of the proper transcription start site, DNA unwinding, and RNAPII promoter escape during transcription (1). During the transition from transcription initiation to elongation, TFIIE stimulates the TFIIH kinase and DNA helicase activities, responsible for phosphorylation of the carboxy-terminal domain of the largest RNAPII subunit (POL2RA) and unwinding of promoter DNA for RNAPII promoter escape (1,5-9).

  1. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.
  2. Ohkuma, Y. et al. (1991) Nature 354, 398-401.
  3. Sumimoto, H. et al. (1991) Nature 354, 401-4.
  4. Peterson, M.G. et al. (1991) Nature 354, 369-73.
  5. Ohkuma, Y. and Roeder, R.G. (1994) Nature 368, 160-3.
  6. Ohkuma, Y. et al. (1995) Mol Cell Biol 15, 4856-66.
  7. Serizawa, H. et al. (1994) J Biol Chem 269, 20750-6.
  8. Larochelle, S. et al. (2012) Nat Struct Mol Biol 19, 1108-15.
  9. Grünberg, S. et al. (2012) Nat Struct Mol Biol 19, 788-96.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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