Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

DMAP1 Antibody #13326

No. Size Price
13326S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13326 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 60 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Zebrafish, Bovine, Dog,

Specificity / Sensitivity

DMAP1 Antibody recognizes endogenous levels of total DMAP1 protein.

DMAP1 Antibody能够检测内源性DMAP1的总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly121 of human DMAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人源DMAP1蛋白的Gly121周围序列相对应的肽段免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DMAP1 Antibody. Western blot方法检测不同细胞系的提取物,使用的抗体为DMAP1 Antibody。

Background

DNA methyltransferase 1 (DNMT1)-associated protein 1 (DMAP1) is a nuclear protein that functions in transcriptional repression and DNA repair. DMAP1 was first identified as an activator of DNMT1 methyltransferase activity (1). Both DMAP1 and DNMT1 are targeted to replication foci during S phase and function to transfer proper methylation patterns to newly synthesized DNA during replication (1). In late S phase, DMAP1-DNMT1 co-operate with a p33ING1-Sin3-HDAC2 complex to maintain pericentric heterochromatin by deacetylating histones, methylating histone H3 at Lys9, and methylating DNA (1,2). The DMAP1 protein is also part of the TIP60-p400 complex, a histone acetyltransferases (HAT) and chromatin-remodeling complex that functions in DNA repair (3,4). Upon DNA damage, the TIP60-p400 complex acetylates histone H4 at Lys16 to induce chromatin relaxation and activation of the ATM kinase. DMAP1 is required for DNA-damage induced TIP60-p400-mediated histone acetylation, and deletion of DMAP1 impairs AMT function (5). DMAP1-DNMT1 may also methylate DNA at sites of DNA damage during homologous recombination, which results in gene silencing (6).

DNA methyltransferase 1 (DNMT1)-associated protein 1 (DMAP1)是一个核蛋白,在转录抑制和DNA修复中发挥作用。DMAP1最早是作为DNMT1甲基转移酶活性的激活因子被发现的(1)。DMAP1和DNMT1都靶向于S期的复制基因座,能够在复制过程中将正确的甲基化形式转移至新和成的DNA上(1)。在S晚期,DMAP1-DNMT1与p33ING1-Sin3-HDAC2复合体协同作用通过将组蛋白去乙酰化,组蛋白H3 Lys9位点的甲基化以及DNA的甲基化来维持臂间异染色质(1,2)。DMAP1蛋白同样也是TIP60-p400符合日的一部分,该复合体是一种histone acetyltransferases (HAT)以及染色质重构复合体,能够在DNA修复中发挥作用(3,4)。一旦发生DNA损伤,TIP60-p400复合体会将组蛋白H4的Lys16位点乙酰化从而引起染色质松弛以及ATM激酶的激活。DNA损伤引起的TIP60-p400介导的组蛋白乙酰化需要DMAP1的参与,DMAP1的缺失会削弱AMT的功能(5)。DMAP1-DNMT1可能也可以在同源重组过程中在DNA损伤位点甲基化DNA,从而导致基因沉默(6)。

  1. Rountree, M.R. et al. (2000) Nat Genet 25, 269-77.
  2. Xin, H. et al. (2004) J Biol Chem 279, 9539-46.
  3. Cai, Y. et al. (2003) J Biol Chem 278, 42733-6.
  4. Doyon, Y. et al. (2004) Mol Cell Biol 24, 1884-96.
  5. Penicud, K. and Behrens, A. (2013) Oncogene , 525-31.
  6. Lee, G.E. et al. (2010) J Biol Chem 285, 37630-40.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications