Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IL-17F (D3M4D) Rabbit mAb (Mouse Specific) #13186

No. Size Price
13186S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13186 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 18 Rabbit IgG
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Mouse IL-17F (D3M4D) Rabbit mAb recognizes endogenous levels of total mouse IL-17F protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser93 of mouse IL-17F protein.

Western Blotting

Western Blotting

Western blot analysis of EL4 cells, untreated (-) or treated with TPA #4174 (50 ng/ml), Ionomycin, Calcium Salt #9995 (500 ng/ml), and Brefeldin A #9972 (500 ng/ml) overnight (+), using Mouse IL-17F (D3M4D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of mouse splenocytes, untreated (blue) or treated with PMA (50 ng/ml), Ionomycin (200 ng/ml), and hTGF-β (10 ng/ml) for 24 hr (green), using Mouse IL-17F (D3M4D) Rabbit mAb compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

The IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE (1). IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells (2). IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides (2). In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases (3). IL-17E promotes Th2 cell responses (4). The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines (1,5,6). IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1 (7). Unlike most cytokines that signal through Jak/STAT pathways, IL-17 signaling results in NF-κB activation (8).

IL-17F is a cysteine-linked proinflammatory cytokine that can dimerize with itself or form heterodimers with the IL-17 family member that it shares 50% homology with, IL-17A (9). Although mainly produced by Th17 cells, IL-17F expression has been observed in several cell types including activated CD8+ T cells, γδ T cells, NKT cells, B cells and LTi cells. IL-17F binds to a heterodimeric receptor consisting of IL-17RA and IL-17RC, which upon binding induces the TRAF6-mediated activation of TAK and the Erk1/2 MAP kinase pathway (10). This induces the expression of numerous inflammatory chemokines and cytokines including IL-1β, IL-6, IL-8, and MIP-1β along with increased adhesion molecule expression in human airway epithelial cells, vein endothelial cells, and fibroblasts (11). IL-17F has been linked with asthma and other autoimmune diseases including rheumatoid arthritis, multiple sclerosis, psoriasis, and inflammatory bowel disease (12).

  1. Gaffen, S.L. (2009) Nat Rev Immunol 9, 556-67.
  2. Iwakura, Y. et al. (2011) Immunity 34, 149-62.
  3. Hu, Y. et al. (2011) Ann N Y Acad Sci 1217, 60-76.
  4. Fort, M.M. et al. (2001) Immunity 15, 985-95.
  5. Yamaguchi, Y. et al. (2007) J Immunol 179, 7128-36.
  6. Li, H. et al. (2000) Proc Natl Acad Sci U S A 97, 773-8.
  7. Chang, S.H. et al. (2006) J Biol Chem 281, 35603-7.
  8. Shalom-Barak, T. et al. (1998) J Biol Chem 273, 27467-73.
  9. Liang, S.C. et al. (2007) J Immunol 179, 7791-9.
  10. Chang, S.H. and Dong, C. (2007) Cell Res 17, 435-40.
  11. Hizawa, N. et al. (2006) Clin Exp Allergy 36, 1109-14.
  12. Isailovic, N. et al. (2015) J Autoimmun 60, 1-11.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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