Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RBBP5 (D3I6P) Rabbit mAb #13171

histone methyltransferase   HMT   MLL   Set1   SetDB  

No. Size Price
13171S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13171 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Horse,

Specificity / Sensitivity

RBBP5 (D3I6P) Rabbit mAb recognizes endogenous levels of total RBBP5 protein.

RBBP5 (D3I6P) Rabbit mAb兔单抗能够检测内源性RBBP5总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val408 of human RBBP5 protein.

该单克隆抗体是经由采用合成的与人源RBBP5蛋白Val408残基周围序列相对应的肽段免疫动物而生产的。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of RBBP5 (D3I6P) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 对4 x 106HeLa细胞中交联过的染色质进行染色质免疫沉淀,所用抗体为10 µl RBBP5 (D3I6P)或2 µl Normal Rabbit IgG #2729,富集到的DNA经过实时PCR定量,所使用产品为SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBBP5 (D3I6P) Rabbit mAb. Western blot方法检测不同细胞系的提取物,使用的抗体为 RBBP5 (D3I6P) Rabbit mAb。

IP

IP

Immunoprecipitation of RBBP5 from C2C12 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RBBP5 (D3I6P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RBBP5 (D3I6P) Rabbit mAb. 从C2C12细胞提取物中免疫沉淀RBBP5,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或RBBP5 (D3I6P) Rabbit mAb (lane 3)。Lane 1为10% input。 使用RBBP5 (D3I6P) Rabbit mAb进行Western blot检测。

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).

Set1组蛋白甲基转移酶最初是在酵母中被首次鉴定出来的,并且是Set1/COMPASS组蛋白甲基转移酶复合物中的一部分,该复合物能偶使histone H3蛋白Lys4位点甲基化并作为转录辅助激活因子起作用(1)。酵母仅含有一个已知的Set1蛋白,而哺乳动物则含有六个Set1相关蛋白:SET1A、SET1B、MLL1、MLL2、MLL3和MLL4,所有这些蛋白都能组装到COMPASS样复合物中,并使组蛋白H3 的Lys4位点甲基化(2,3)。这些Set1相关蛋白中的每一个都是在不同的蛋白质复合物被发现的,他们都含有共同亚基WDR5、RBBP5、ASH2L、CXXC1和DPY30,而这些亚基是复合物的正确组装和组蛋白甲基转移酶活性调节所需要的(2-6)。MLL1和MLL2复合物含有另外一个蛋白亚基menin (6)。像酵母Set1一样,所有六种Set1相关哺乳动物蛋白可以使组蛋白 H3 Lys4位点甲基化(2-6)。许多血液恶性肿瘤种都发现有MLL易位,这暗示着Set1/COMPASS组蛋白甲基转移酶复合物在白血病生成中有至关重要的作用 (6)。

  1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
  2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
  3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
  4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
  5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
  6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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