Cell Signaling Technology

Product Pathways - DNA Damage

p63-α (D2K8X) XP® Rabbit mAb #13109

No. Size Price
13109S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
13109T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
13109 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 75 Rabbit IgG
IP 1:100
F 1:800
IF-IC 1:800
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

p63-α (D2K8X) XP® Rabbit mAb recognizes endogenous levels of total p63-α protein. This antibody will detect both the full-length isoform (TAp63-α) and the truncated δNp63-α isoform, but will not recognize the p63-β or p63-γ isoform. Rabbit mAb可以识别内源性p63-α总蛋白。该抗体可以检测全长异构体(TAp63-α)和截短的δNp63-α异构体,但是不能识别p63-β或 p63-γ isoform.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p63-α protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人p63-α蛋白的羧基端一致。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of ME-180 (green) and MCF7 (blue) cells using p63-α (D2K8X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用p63-α (D2K8X) XP® Rabbit mAb对ME-180(绿色)和MCF7(蓝色)细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412用作二抗。



Confocal immunofluorescent analysis of ME-180 (left) and MCF7 (right) cells using p63-α (D2K8X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用p63-α (D2K8X) XP® Rabbit mAb(绿色)对ME-180 (左)和 MCF7 (右)细胞进行激光共聚焦免疫荧光分析。使用DY-554鬼笔环肽(红)标记肌动蛋白丝。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from ME-180 (+), HaCaT (+), and MCF7 (-) cells using p63-α (D2K8X) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).使用p63-α (D2K8X) XP®Rabbit mAb(上)或β-Actin (D6A8) Rabbit mAb #8457(下)对ME-180 (+), HaCaT (+), 和MCF7 (-)细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HaCaT cells and either 5 μl of p63-α (D2K8X) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.



Immunoprecipitation of p63-α from Me180 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p63-α (D2K8X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p63-alpha (D2K8X) XP® Rabbit mAb.


The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4).

p53抑癌蛋白在细胞对DNA损伤和其他基因组畸变过程中发挥了重要作用。激活p53将导致细胞周期停滞和DNA修复或凋亡(1)。除了p53,哺乳动物细胞还含有2种p53家族成员,p63和p73,它们与p53在结构和功能上都很类似(2)。p63可以诱导p53-响应基因和凋亡,p63突变很少会导致肿瘤(2)。研究人员经常能在肺,头部和颈部鳞癌细胞中检测到p63基因的增殖(2,3)。p63基因含有一个选择性的转录起始位点会产生一个缺失转录激活结构域的40kDa δNp63,羧基端的选择性剪切会产生α, β, 和γ三种异构体(3,4)。

  1. Levine, A.J. (1997) Cell 88, 323-31.
  2. Waltermann, A. et al. (2003) Oncogene 22, 5686-93.
  3. Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7.
  4. Yang, A. et al. (1999) Nature 398, 714-8.

Application References

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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