Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

NVP-BEZ235 #13101

BEZ235   CDK4   cyclin D3   inhibitor   mTOR   NVP   PI3K  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Molecular Weight:

469.54 g/mol

Molecular Characterization

C30H23N5O

Purity

>99%

Bioactivity

Solubility: Soluble in DMF at 10 mg/ml with warming. Soluble in DMSO at 1.33 mg/ml with warming. Very poorly soluble in water and ethanol.

Structure

Structure

Chemical structure of NVP-BEZ235.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with NVP-BEZ235 (2 hr) at the indicated concentrations. The phosphorylation of p70 S6 kinase was detected using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper) and p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with NVP-BEZ235 (2 hr) at the indicated concentrations. The phosphorylation of Akt was detected using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Directions for Use

NVP-BEZ235 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.065 ml DMF. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 5 nM-500 nM for 2-24 hr.

Background

NVP-BEZ235 is a selective and potent dual inhibitor of PI3 kinase and mTOR Ser/Thr protein kinase. Research studies show that NVP-BEZ235 reversibly inhibits the catalytic activity of these kinases by competing with ATP at the ATP-binding site (1,2). Studies demonstrate that NVP-BEZ235 has anti-proliferative characteristics and induces cell cycle arrest during G0/G1 phase through inhibition of CDK4 and cyclin D3 (2,3).

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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