Product Pathways - PI3K / Akt Signaling
|13101S||5 mg||￥1,543.00 现货查询||购买询价|
|13101||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Solubility: Soluble in DMF at 10 mg/ml with warming. Soluble in DMSO at 1.33 mg/ml with warming. Very poorly soluble in water and ethanol.
Western blot analysis of extracts from Jurkat cells, untreated or treated with NVP-BEZ235 (2 hr) at the indicated concentrations. The phosphorylation of p70 S6 kinase was detected using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper) and p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower).
Directions for Use
NVP-BEZ235 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.065 ml DMF. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 5 nM-500 nM for 2-24 hr.
NVP-BEZ235 is a selective and potent dual inhibitor of PI3 kinase and mTOR Ser/Thr protein kinase. Research studies show that NVP-BEZ235 reversibly inhibits the catalytic activity of these kinases by competing with ATP at the ATP-binding site (1,2). Studies demonstrate that NVP-BEZ235 has anti-proliferative characteristics and induces cell cycle arrest during G0/G1 phase through inhibition of CDK4 and cyclin D3 (2,3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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