Cell Signaling Technology

Product Pathways - Protein Stability

UBE2T (D2L7H) Rabbit mAb #12992

UBC   UBE2   Ubiquitin Conjugating Enzymes  

No. Size Price
12992S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12992 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 25 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

UBE2T (D2L7H) Rabbit mAb recognizes endogenous levels of total UBE2T protein.

UBE2T (D2L7H) Rabbit mAb兔单抗识别内源性的水的UBE2T总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His150 of human UBE2T protein.

此单克隆抗体经合成与人UBE2T蛋白His150邻近的氨基酸残基序列一致的肽段,免疫动物而产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBE2T (D2L7H) Rabbit mAb.Western blot检测多种细胞提取物,使用的抗体是UBE2T (D2L7H) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2T (hUBE2T-Myc/DDK; +), using UBE2T (D2L7H) Rabbit mAb.Western blot分析293T细胞提取物,对照未转染(-)或转染了Myc/DDK标签的全长人源的UBE2T (hUBE2T-Myc/DDK; +),使用的抗体是UBE2T (D2L7H) Rabbit mAb。

IP

IP

Immunoprecipitation of UBE2T from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UBE2T (D2L7H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE2T (D2L7H) Rabbit mAb.对293细胞提取物中的UBE2T进行免疫共沉淀,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或UBE2T (D2L7H) Rabbit mAb (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是UBE2T (D2L7H) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® UBE2T siRNA I #13142 (+), using UBE2T (D2L7H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The UBE2T (D2L7H) Rabbit mAb confirms silencing of UBE2T expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot检测293T细胞提取物,转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® UBE2T siRNA I #13142 (+),使用的抗体是UBE2T (D2L7H) Rabbit mAb (上图)或GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。UBE2T (D2L7H) Rabbit mAb证实了UBE2T沉默表达,采用GAPDH (D16H11) XP® Rabbit mAb做对照。

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

Ubiquitin conjugating-enzyme 2T (UBE2T) is an E2 family member responsible for the ATP-dependent ubiquitin tagging of target proteins for degradation. Research studies indicate that UBE2T plays an important role in the Fanconi anemia pathway and that UBE2T expression is required for normal DNA repair through this pathway. Interaction between UBE2T and FANCL appears to stimulate UBE2T auto monoubiquitination, leading to UBE2T inactivation and negative regulation of the Fanconi anemia pathway (2-4). Additional research details upregulation of UBE2T expression in breast cancer cells and certain lung carcinomas, suggesting a possible involvement in these malignancies (5,6).

蛋白泛素化作用需要E1, E2, 和E3泛素-连接酶的协同作用。泛素最先通过具有硫酯的泛素激活酶E1通过ATP-依赖形式活化。活化的泛素然后通过含有巯基的泛素载体蛋白E2转运。最后从E2的泛素转运到含有ε-氨基的赖氨酸残疾靶蛋白,这由泛素连接酶E3介导(1)。

UBE2T是E2家族成员,负责ATP依赖性的蛋白质降解。研究发现UBE2T在范科尼贫血中扮演重要角色,并且通过这种途径参与一般的DNA修复过程。UBE2T与FANCL的相互作用激活UBE2T自单泛素化,导致UBE2T的失活和范科尼贫血的负调控(2-4)。另外研究表明, 在乳腺癌和一些肺癌中UBE2T的表达上调,表明其可能参与肿瘤恶化(5,6)。

  1. Hershko, A. (1988) J Biol Chem 263, 15237-40.
  2. Machida, Y.J. et al. (2006) Mol Cell 23, 589-96.
  3. Ramaekers, C.H. et al. (2011) Radiother Oncol 101, 190-7.
  4. Zhang, Y. et al. (2007) J Genet Genomics 34, 573-80.
  5. Ueki, T. et al. (2009) Cancer Res 69, 8752-60.
  6. Hao, J. et al. (2008) Tumour Biol 29, 195-203.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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