Cell Signaling Technology

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Western Blotting Application Solutions Kit #12957

13005   13131   companion   complementary   kit   reagent   WB   WB Kit   western blot   western blot kit   western blotting   western blotting kit   western kit   westerns  

REACTIVITY

No. Size Price
12957S 1 Kit ( 10 western blots ) ¥4,996.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
Nitrocellulose Sandwiches #12369 10 Pack
Tris-Glycine Transfer Buffer (10X) #12539 300 ml
Prestained Protein Marker, Broad Range (11-190 kDa) #13953 100 µl
Tris-Glycine SDS Running Buffer (10X) #4050 300 ml
SignalFire™ ECL Reagent #6883 100 ml
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse
Blue Loading Buffer Pack #7722 1 Pack
PMSF #8553 34.84 mg 174.19
Cell Lysis Buffer (10X) #9803 15 ml
Tris Buffered Saline with Tween® 20 (TBST-10X) #9997 300 ml
BSA #9998 12 g
Nonfat Dry Milk #9999 50 g

Description

The Western Blotting Application Solutions Kit is designed to conveniently provide reagents needed for western blotting, from cell lysis to protein detection. The reagents in this kit are thoroughly validated with our primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents to run 10 mini-gels and complete western blot assays with either rabbit or mouse primary antibodies. All reagents in this kit are available individually.

Bioactivity

Storage:

t

Recommended Secondary Antibody Dilutions:

Anti-rabbit IgG, HRP-linked Antibody #7074 1:1000-1:3000

Anti-mouse IgG, HRP-linked Antibody #7076 1:1000-1:3000

Western Blotting

Western Blotting

10-20% SDS-PAGE band profile of the Prestained Protein Marker, Broad Range (Premixed Format) #7720.

Western Blotting

Western Blotting

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

SignalFire is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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