Product Pathways - Companion Products
Western Blotting Application Solutions Kit #12957
|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Nitrocellulose Sandwiches #12369||10 Pack|
|Tris-Glycine Transfer Buffer (10X) #12539||300 ml|
|Prestained Protein Marker, Broad Range (11-190 kDa) #13953||100 µl|
|Tris-Glycine SDS Running Buffer (10X) #4050||300 ml|
|SignalFire™ ECL Reagent #6883||100 ml|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
|Blue Loading Buffer Pack #7722||1 Pack|
|PMSF #8553||34.84 mg||174.19|
|Cell Lysis Buffer (10X) #9803||15 ml|
|Tris Buffered Saline with Tween® 20 (TBST-10X) #9997||300 ml|
|BSA #9998||12 g|
|Nonfat Dry Milk #9999||50 g|
The Western Blotting Application Solutions Kit is designed to conveniently provide reagents needed for western blotting, from cell lysis to protein detection. The reagents in this kit are thoroughly validated with our primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents to run 10 mini-gels and complete western blot assays with either rabbit or mouse primary antibodies. All reagents in this kit are available individually.
Recommended Secondary Antibody Dilutions:
Anti-rabbit IgG, HRP-linked Antibody #7074 1:1000-1:3000
Anti-mouse IgG, HRP-linked Antibody #7076 1:1000-1:3000
10-20% SDS-PAGE band profile of the Prestained Protein Marker, Broad Range (Premixed Format) #7720.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12498 Tris Buffered Saline (TBS-10X)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7780 BCA Protein Assay Kit
- 9808 Phosphate Buffered Saline (PBS-20X)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
SignalFire is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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