Cell Signaling Technology

Product Pathways - Metabolism

Glut1 (D3J3A) Rabbit mAb #12939

DYT17   DYT18   Glucose transporter type 1. erythrocyte/brain   GLUT   GLUT-1   GLUT1   GTR1   HepG2 glucose transporter   MGC141895   MGC141896   PED   sc-7903   SLC2A1   solute carrier family 2 (facilitated glucose transporter). member 1   Solute carrier family 2. facilitated glucose transporter member 1  

No. Size Price
12939S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12939 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 45-60 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Glut1 (D3J3A) Rabbit mAb recognizes endogenous levels of total Glut1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu260 of human Glut1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Glut1 (D3J3A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Glut1 siRNA I (+) #14184, using Glut1 (D3J3A) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IP

IP

Immunoprecipitation of Glut1 from Huh6 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Glut1 (D3J3A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Glut1 (D3J3A) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 was used as the secondary antibody.

Background

Glucose transporter 1 (Glut1, SLC2A1) is a widely expressed transport protein that displays a broad range of substrate specificity in transporting a number of different aldose sugars as well as an oxidized form of vitamin C into cells (1,2). Glut1 is responsible for the basal-level uptake of glucose from the blood through facilitated diffusion (2). Research studies show that Glut1 and the transcription factor HIF-1α mediate the regulation of glycolysis by O-GlcNAcylation in cancer cells (3). Additional studies demonstrate that Glut1 is required for CD4 T cell activation and is critical for the expansion and survival of T effector (Teff) cells (4). Mutations in the corresponding SLC2A1 gene cause GLUT1 deficiency syndromes (GLUT1DS1, GLUT1DS2), a pair of neurologic disorders characterized by delayed development, seizures, spasticity, paroxysmal exercise-induced dyskinesia, and acquired microcephaly (5,6). Two other neurologic disorders - dystonia-9 (DYT9) and susceptibility to idiopathic generalized epilepsy 12 (EIG12) - are also caused by mutations in the SLC2A1 gene (7,8).

  1. Ferrer, C.M. et al. (2014) Mol Cell 54, 820-31.
  2. Deng, D. et al. (2014) Nature 510, 121-5.
  3. Agus, D.B. et al. (1997) J Clin Invest 100, 2842-8.
  4. Macintyre, A.N. et al. (2014) Cell Metab 20, 61-72.
  5. Wang, D. et al. (2005) Ann Neurol 57, 111-8.
  6. Schneider, S.A. et al. (2009) Mov Disord 24, 1684-8.
  7. Weber, Y.G. et al. (2011) Neurology 77, 959-64.
  8. Suls, A. et al. (2009) Ann Neurol 66, 415-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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