Cell Signaling Technology

Product Pathways - Screening Technologies

K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate) #12930

K-63   K63   K63-linked   K63-ub   ub   ubiquitin  

No. Size Price
12930S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
12930 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate) detects polyubiquitin chains formed by Lys63 residue linkage. It does not react with monoubiquitin or polyubiquitin chains formed by linkage to a different lysine residue.

K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP 偶联)兔单抗识别Lys63位偶联的多泛素化链。这一抗体与单泛素和其他残基偶联的多泛素链无交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Lys63 branch of the human diubiquitin chain.

此单克隆抗体通过合成与人双泛素链的Lys63位分枝序列一致的肽段,免疫动物获得。

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb #5621.

CST公司的这个抗体通过它的胺基团与HRP的碳水化合物结合。预测此HRP偶联的抗体与未结合的K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb #5621一样的同种之间的交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with the proteasome inhibitor MG-132 #2194 (10 µM, 6 hr; +), using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate).Western blot分析HeLa细胞提取物,未处理(-)或蛋白质酶体抑制剂处理MG-132 #2194(10uM, 6小时;+),使用的抗体是K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate)。

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

泛素是一种保守的多肽单元,在泛素蛋白酶体通路中起重要作用。泛素能与许多细胞蛋白共价连接,并被26S蛋白酶降解。3种组分包含在靶蛋白泛素共价结合过程。泛素首先在具有泛素活化酶(UBE1 or E1)的硫酯复合物的形成中被活化。活化的泛素随后被转运到泛素载体蛋白(共价结合酶)E2,随后为了最后的靶蛋白赖氨酸残基的epsilon氨基,从E2到泛素连接酶E3(1-3)。泛素蛋白酶体通路在正常生物过程和疾病中广泛存在。一些蛋白,诸如IκB, p53, cdc25a, 和Bcl-2,是泛素-蛋白酶体过程的靶蛋白,并可以调节细胞周期进展、分化、细胞压力应答以及凋亡(4-7)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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