Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

PAF1 (D9G9X) Rabbit mAb #12883

No. Size Price
12883S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12883 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 80 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig, Horse,

Specificity / Sensitivity

PAF1 (D9G9X) mAb recognizes endogenous levels of total PAF1 protein.

PAF1 (D9G9X) mAb兔单抗能够检测内源性 PAF1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp45 of human PAF1 protein.

该单克隆抗体是采用合成的与人源PAF1蛋白的Asp45残基周围序列相对应的肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PAF1 (D9G9X) Rabbit mAb. Western blot方法检测不同细胞系的提取物,使用的抗体为

Background

The PAF (RNA polymerase II (RNAPII) associated factor) complex was initially identified in yeast and is comprised of subunits PAF1, Leo1, Ctr9, Cdc73, RTF1 and Ski8 (1,2). The PAF complex plays an important role in transcription initiation and elongation by RNAPII by regulating the establishment of proper histone modifications such as histone H2B ubiquitination and the recruitment of the histone chaperone FACT (facilitates chromatin transcription) (3-5). The PAF complex also plays a role in mRNA processing and maturation by interacting with and recruiting the cleavage and polyadenylation specificity factor and cleavage stimulation factor complexes via the Cdc73 subunit (6,7). In addition, the Ski8 subunit of the PAF complex is part of the hSKi complex that regulates RNA surveillance, suggesting an important function of the complex in coordinating events associated with proper RNA maturation during transcription (1,8).

PAF (RNA polymerase II (RNAPII) associated factor)复合物最初是在酵母中被确认的,由亚基PAF1, Leo1, Ctr9, Cdc73, RTF1 以及 Ski8 组成(1,2)。PAF复合体可以调控建立正确的组蛋白修饰如组蛋白H2B泛素化以及招募组蛋白伴侣FACT (facilitates chromatin transcription)从而在RNAPII介导的转录起始和延伸过程中扮演重要角色(3-5)。PAF复合体能够通过Cdc73亚基募集切割和多聚腺苷酸化特异性因子以及切割刺激因子复合体并与之相互作用,从而在mRNA 加工以及成熟过程中发挥功能(6,7)。此外,PAF 复合体的Ski8亚基是hSKi符合日的一部分,能够调控RNA监控,这意味着复合体对于转录过程中与正确的RNA成熟相关事件的调控中发挥重要功能。

  1. Jaehning, J.A. (2010) Biochim Biophys Acta 1799, 379-88.
  2. Chaudhary, K. et al. (2007) Oncogene 26, 7499-507.
  3. Pavri, R. et al. (2006) Cell 125, 703-17.
  4. Rozenblatt-Rosen, O. et al. (2005) Mol Cell Biol 25, 612-20.
  5. Zhu, B. et al. (2005) Genes Dev 19, 1668-73.
  6. Rozenblatt-Rosen, O. et al. (2009) Proc Natl Acad Sci U S A 106, 755-60.
  7. Farber, L.J. et al. (2010) Mol Carcinog 49, 215-23.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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