Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

POLR3A (D5Y2D) Rabbit mAb #12825

No. Size Price
12825S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12825 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 165 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Bovine, Dog, Guinea Pig,

Specificity / Sensitivity

POLR3A (D5Y2D) Rabbit mAb recognizes endogenous levels of total POLR3A protein.

POLR3A (D5Y2D) Rabbit mAb 兔单抗能够检测内源性POLR3A总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val613 of human POLR3A protein.

该多克隆抗体是采用合成的与人源POLR3A蛋白Val613 残基周围序列相对应的肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using POLR3A (D5Y2D) Rabbit mAb.Western blot 方法检测不同细胞系的提取物,使用的抗体为POLR3A (D5Y2D) Rabbit mAb

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of POLR3A (D5Y2D) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human L1M5 LINE element primers, SimpleChIP® Human tRNA-Leu Anti-Codon (TAG) Primers #12769, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 HeLa细胞中提取的交联过的染色质,以及10 µl POLR3A (D5Y2D) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量,使用引物为人L1M5 LINE 元件引物,SimpleChIP® Human tRNA-Leu Anti-Codon (TAG) Primers #12769以及 SimpleChIP® Human α Satellite Repeat Primers #4486.。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。


POLR3A is the largest subunit of the DNA-dependent RNA polymerase III, one of the DNA-dependent RNA polymerases that transcribe small non-coding RNAs such as the 5S rRNA, tRNAs and some miRNAs (1-3). Together with the second largest subunit, POLR3A forms the catalytic center of the polymerase (4). In addition, RNA polymerase III plays a role in the innate immune response by sensing non-self double stranded DNA. Transcription of the non-self DNA into RNA induces type I interferon production through the RIG-I pathway (5,6). Studies suggest that mutations in the POLR3A gene may be linked to hypomyelinating leukodystrophies; a group of inherited neurodegenerative disorders (7-9).

POLR3A是DNA依赖的RNA聚合酶 III的最大亚基。此聚合酶是DNA依赖的RNA聚合酶的一种,能够转录小的非编码RNA,如5S rRNA, tRNAs 以及 一些 miRNA (1-3)。POLR3A与第二大的亚基一起形成了聚合酶的催化中心(4)。此外,RNA聚合酶III可感知异己的双链DNA从而在天然免疫反应中发挥作用。,异己DNA转录到RNA可通过RIG-1信号通路降低I型白介素的生产(5,6)。研究标明POLR3A基因的突变可能与一类遗传神经退行性疾病有关,比如髓鞘形成不足导致的脑白质病变(7-9)。

  1. Dieci, G. et al. (2007) Trends Genet 23, 614-22.
  2. Teichmann, M. et al. (2010) Transcription 1, 130-135.
  3. White, R.J. (2011) Nat Rev Genet 12, 459-63.
  4. Werner, M. et al. (2009) Curr Opin Struct Biol 19, 740-5.
  5. Chiu, Y.H. et al. (2009) Cell 138, 576-91.
  6. Ablasser, A. et al. (2009) Nat Immunol 10, 1065-72.
  7. Saitsu, H. et al. (2011) Am J Hum Genet 89, 644-51.
  8. Bernard, G. et al. (2011) Am J Hum Genet 89, 415-23.
  9. Daoud, H. et al. (2013) J Med Genet 50, 194-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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