Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCC2/BAF170 (D8O9V) Rabbit mAb #12760

No. Size Price
12760S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12760 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 162, 170 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Dog, Pig, Guinea Pig, Horse,

Specificity / Sensitivity

SMARCC2/BAF170 (D8O9V) Rabbit mAb recognizes endogenous levels of total SMARCC2/BAF170 protein.

SMARCC2/BAF170 (D8O9V) Rabbit mAb兔单抗检测内源性SMARCC2/BAF170总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile818 of human SMARCC2/BAF170 protein.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells, grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min), and either 10 μl of SMARCC2/BAF170 (D8O9V) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 MCF7细胞中提取的交联过的染色质,以及10 µl SMARCC2/BAF170 (D8O9V) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀,这些细胞需要在含5%活性炭处理胎牛血清以及无酚红的培养基中培养4天,之后再用β-estradiol 进行处理(10 nM, 45 min)。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量,所使用引物为SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, 以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMARCC2/BAF170 (D8O9V) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为SMARCC2/BAF170 (D8O9V) Rabbit mAb。



Immunoprecipitation of SMARCC2/BAF170 from PANC-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SMARCC2/BAF170 (D8O9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SMARCC2/BAF170 (D8O9V) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀SMARCC2/BAF170,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或SMARCC2/BAF170 (D8O9V) Rabbit mAb (lane 3)。Lane 1为10% input。 使用SMARCC2/BAF170 (D8O9V)进行Western blot检测。


ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

ATP依赖的染色质改构复合物在转录、DNA复制和修复等核进程的调控中扮演着重要角色。(1,2)。SWI/SNF染色质改构复合物由超过10个亚单位组成,其中包含一个单分子的BRM 和BRG1作为ATPase的催化亚基。ATPase亚单位激活后会破坏组蛋白-DNA的接触,并改变决定性调控原色对染色质的可接近性。另外的核心亚基和配体亚基作为骨架以维持其稳定性同时还可作为其与不同转录因子及染色质相互作用的接触界面(2,5)。SWI/SNF与核受体、p53、Rb、BRCA1以及MyoD等转录因子的相互作用能够促进复合体募集到目的基因上以调控基因激活,细胞生长,细胞周期以及分化进程(1,6-9)。

SMARCC2/BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 in vitro (10). While SMARCC2/BAF170 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Research studies have shown that expression of SMARCC2/BAF170 is up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2/BAF170 leads to loss of stem cell pluripotency and self renewal (11).


  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
  11. Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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