Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HMGN1 (D1I5O) Rabbit mAb #12734

No. Size Price
12734S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12734 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 18 Rabbit IgG
IP 1:50
IF-IC 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

HMGN1 (D1I5O) Rabbit mAb recognizes endogenous levels of total HMGN1 protein. This antibody does not cross-react with other HMGN proteins.

HMGN1 (D1I5O) Rabbit mAb兔单抗能够检测内源性HMGN1总蛋白水平。该抗体不会与其它HMGN蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val32 of human HMGN1 protein.

该单克隆抗体是采用合成的围绕人HMGN1蛋白Val32残基周围序列相对应的肽段免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCCIT cells using HMGN1 (D1I5O) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测NCCIT细胞,使用的抗体为HMGN1 (D1I5O) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HMGN1 (D1I5O) Rabbit mAb.Western blot方法检测不同细胞系的提取物,使用的抗体为HMGN1 (D1I5O) Rabbit mAb。

IP

IP

Immunoprecipitation of HMGN1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HMGN1 (D1I5O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HMGN1 (D1I5O) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀HMGA1,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或HMGN1 (D1I5O) Rabbit mAb (lane 2)。Lane 1为10% input。 使用HMGN1 (D1I5O) Rabbit mAb进行Western blot检测。

Background

High mobility group (HMG) proteins are a superfamily of abundant and ubiquitous nuclear proteins that bind DNA without sequence specificity and induce structural changes to the chromatin fiber to regulate access to the underlying DNA. The HMGN family of proteins, which includes five members (HMGN1-5), is characterized by the presence of several conserved protein domains: a positively charged domain, a nucleosome binding domain, and an acidic C-terminal chromatin-unfolding domain (1,2). HMGN proteins function in transcriptional regulation and are recruited to gene promoters by transcription factors, such as estrogen receptor α (ERα), serum responsive factor (SRF), and PITX2, where they can facilitate either gene activation or repression (3-5). HMGN proteins bind specifically to nucleosomal DNA and reduce compaction of the chromatin fiber, in part by competing with linker histone H1 for nucleosome binding (6). In addition, HMGN proteins act to modulate local levels of post-translational histone modifications, decreasing phosphorylation of histone H3 at Ser10 and histone H2A at Ser1 and increasing acetylation of histone H3 at Lys14 (7-9). HMGN proteins can also modulate the activity of several chromatin-remodeling factors and restrict nucleosome mobility (10).

High mobility group (HMG)蛋白是一个大量和广泛的细胞核蛋白超级家族,该家族在没有序列特异性的情况下结合DNA,并且诱导染色质纤维的结构性改变从而调节潜在的DNA通路。HMGN 蛋白家族包括五种成员(HMGN1-5),它是以数种保守蛋白结构域存在为特征:一个正电荷区域、一个核小体结合区域和一个酸性的C端染色质-非折叠区域(1,2)。HMGN蛋白具有转录调节的功能,并且通过转录因子被招募到基因启动子,例如 estrogen receptor alpha (ERα)、serum responsive factor (SRF)和PITX2蛋白,在启动子区域能有助于基因的激活或抑制(3-5)。HMGN蛋白特异性结合到核小体结构的DNA,并且减少染色质纤维的压缩,对于核小体的结合部分程度上通过竞争histone H1(6)。此外,HMGN蛋白可以调节翻译后组蛋白修饰的局部水平,这减少了histone H3蛋白Ser10位点和histone H2A蛋白Ser1位点的磷酸化,以及增加了histone H3蛋白Lys14位点的乙酰化(7-9)。HMGN蛋白也能调节数个染色质重塑因子的活性和限制核小体移动(10)。

  1. Hock, R. et al. (2007) Trends Cell Biol 17, 72-9.
  2. Gerlitz, G. Biochim Biophys Acta 1799, 80-5.
  3. Zhu, N. and Hansen, U. (2007) Mol Cell Biol 27, 8859-73.
  4. Amen, M. et al. (2008) Nucleic Acids Res 36, 462-76.
  5. Belova, G.I. et al. (2008) J Biol Chem 283, 8080-8.
  6. Catez, F. et al. (2002) EMBO Rep 3, 760-6.
  7. Lim, J.H. et al. (2005) EMBO J 24, 3038-48.
  8. Lim, J.H. et al. (2004) Mol Cell 15, 573-84.
  9. Postnikov, Y.V. et al. (2006) Biochemistry 45, 15092-9.
  10. Rattner, B.P. et al. (2009) Mol Cell 34, 620-6.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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