Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

MERIT40 (D7Y5K) Rabbit mAb #12711

BABAM1  

No. Size Price
12711S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12711 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MERIT40 (D7Y5K) Rabbit mAb recognizes endogenous levels of total MERIT40 protein.MERIT40 (D7Y5K)Rabbit mAb可以识别内源性总的MERIT40蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MERIT40 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人MERIT40蛋白的羧基端一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MERIT40 (D7Y5K) Rabbit mAb.使用MERIT40 (D7Y5K)Rabbit mAb对多种细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of MERIT40 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MERIT40 (D7Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MERIT40 (D7Y5K) Rabbit mAb. A light-chain specific secondary antibody was used.使用(DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或MERIT40 (D7Y5K) Rabbit mAb (lane 3)对HeLa细胞提取物的MERIT40进行免疫沉淀实验。Lane1的上样量是10%。MERIT40 (D7Y5K) Rabbit mAb westerrn blot实验。使用了一种轻链特异性二抗。

Background

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair. BRCA1 forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for the regulation of BRCA1 function. The BRCA1-Rap80 complex (BRCA1 A complex), including Rap80, BRCC36, BRCC45, Abraxas, and MERIT40/NBA1, functions in G2/M phase checkpoint control (reviewed in 1,2). 
 MERIT40/NBA1 localizes to sites of DNA damage and is required for the appropriate localization of BRCA1 in response to ionizing radiation, as well as maintenance of the BRCA1 A complex (3,4). Proteomics studies have identified Ser29 as a phosphorylated site on MERIT40/NBA1, and the significance of this phosphorylation is under investigation (5-9).乳腺癌易感基因,BRCA1,编码一种E3泛素连接酶,BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。BRCA1会和其他DNA损伤蛋白结合形成至少三种不同的复合物(BRCA1A,B和C),这种结合对于调控BRCA1的功能是至关重要的。BRCA1-Rap80 复合物 (BRCA1 A复合物),包括Rap80, BRCC36, BRCC45, Abraxas, 和MERIT40/NBA1,调控G2/M周期检验点(1,2)。MERIT40/NBA1定位于DNA损伤的部位,对于维持电离辐射后引发的BRCA1反应及其正确定位,以及维持BRCA1复合物都是必须的(3.4)。蛋白组学研究发现MERIT40/NBA1的29位丝氨酸可以被磷酸化,该磷酸化的重要性尚待研究(5-9)。

  1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
  2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
  3. Wang, B. et al. (2009) Genes Dev 23, 729-39.
  4. Shao, G. et al. (2009) Genes Dev 23, 740-54.
  5. Moritz, A. et al. (2010) Sci Signal 3, ra64.
  6. Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
  7. Iliuk, A.B. et al. (2010) Mol Cell Proteomics 9, 2162-72.
  8. Wu, F. et al. (2010) Mol Cell Proteomics 9, 1616-32.
  9. Mayya, V. et al. (2009) Sci Signal 2, ra46.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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