Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit #12704

REACTIVITY

No. Size Price
12704T 1 Kit ( 4 x 20 µl ) ¥3,876.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818 20 µl W,IP,IHC-P,IF-IC, H,M,R, 280 Rabbit IgG
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 20 µl W,IHC-P,IF-IC,F, H,M,R,Hm, 280 Rabbit IgG
Acetyl-CoA Carboxylase 1 Antibody #4190 20 µl W,IP, H,M,R, 265 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl W, Goat
Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578 20 µl W,IP, H, 280 Rabbit IgG

Specificity / Sensitivity

Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

每个抗体都可以识别其内源性的靶蛋白。Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 可以识别Ser79被磷酸化的内源性acetyl-CoA carboxylase。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.

合成对应human acetyl-CoA carboxylase 1蛋白序列的肽段免疫动物获得多抗。多抗经过蛋白A和肽段亲和层析纯化获得。合成磷酸肽段免疫动物获得单抗,磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79及其邻近氨基酸序列一致,或和human acetyl-CoA carboxylase α1蛋白的Ser523或human acetyl-CoA carboxylase 2的Val1416获得单抗,磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79。

Description

The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform four western blot experiments per primary antibody.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler试剂盒提供了一种经济的方式以区分两种acetyl-CoA carboxylase亚型,以及total acetyl-CoA carboxylase 和 phosphorylated acetyl-CoA carboxylase。该试剂盒提供了足以进行4次western blot分析的一抗。

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Acetyl-CoA Carboxylase 1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCC827 (left) and C2C12 cells (right) using Acetyl-CoA Carboxylase 1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with either SignalSilence® ACC1 siRNA or SignalSilence® ACC2 siRNA, using Acetyl-CoA Carboxylase 1 Antibody and β-Actin (13E5) Rabbit mAb #4970.

Western Blotting

Western Blotting

Western blot analysis of extracts from human adipocytes using Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Acetyl-CoA Carboxylase 1 Antibody #4190.

使用Acetyl-CoA Carboxylase 1 Antibody #4190对多种细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676.

使用Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676对多种细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with Oligomycin #9996 (0.5 μM, 30 min; +), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818 (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

未处理(-)或经过Oligomycin #9996 处理(0.5 μM, 30 min; +)的SH-SY5Y细胞提取物,使用Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 #11818 (上) 或Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676 (下)进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from human adipocytes using Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578.使用Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578对人脂肪细胞提取物进行western blot分析。

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

乙酰辅酶A羧化酶(ACC)催化乙酰辅酶A羧化形成丙二酰辅酶A(1)。这是脂肪酸生物合成和氧化过程中的关键酶(1)。在啮齿类动物中,脂肪组织中主要表达的是265 kDa的ACC1(ACCα),而280 kDa的ACC2(ACCβ)是氧化组织中的主要构型(1,2)。然而,在人体中,ACC2是在脂肪和氧化组织均是主要构型(1,2)。AMPK催化的Ser79磷酸化与PKA催化的 Ser1200磷酸化会抑制ACC的酶活性(3)。ACC是抗肥胖药物的潜在靶点(4,5)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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