Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RAD21 (D5Y8S) Rabbit mAb #12673

cohesin   cohesion   Nuclear matrix protein 1   NXP-1   sc-166973   sc-98784   SCC1 homolog  

No. Size Price
12673S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12673 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 130 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Dog, Guinea Pig, Horse,

Specificity / Sensitivity

RAD21 (D5Y8S) Rabbit mAb recognizes endogenous levels of total RAD21 protein.

RAD21 (D5Y8S) Rabbit mAb兔单抗能够识别内源性RAD21总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp213 of human RAD21 protein.

该单克隆抗体是采用合成的与人源RAD21蛋白 Asp213位点的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RAD21 (D5Y8S) Rabbit mAb. Western blot方法检测不同细胞系的提取物,使用的抗体为RAD21 (D5Y8S) Rabbit mAb。

Background

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).

黏着复合体由一个SMC1 (SMC1A or B) 和SMC3形成的异源二聚体组成,与另外的RAD21 和STAG proteins (STAG1, 2, or 3)相邻(1,2)。这些蛋白形成一个指环样结构,能够介导S期 DNA复制后两个姐妹染色单体的黏连(1,2)。在前期,RAD21和STAG2被Polo-like kinase (PLK)磷酸化,从而导致黏着复合体从染色体臂上解离;但是,粘着蛋白仍然与着丝粒相连直到后期(3,4)。在后期RAD21被separin/ESPL1切割,从而导致剩下的粘着蛋白从着丝粒上解离,以使有丝分裂过程中姐妹染色单体分离(5)。在细胞凋亡过程中,RAD21被caspase-3和 caspase-7切割,产生一个64 kDa 羧基末端切割产物并能被转移到胞质,可能有助于触发细胞凋亡(6,7)。除了介导姐妹染色单体的黏着,黏着复合体在基因调控和DNA修复中也扮演着重要角色,因为发生DNA损伤时SMC1 和 SMC3都能够被ATM 和ATR激酶磷酸化(1,2)。

  1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
  2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
  3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
  4. Hauf, S. et al. (2005) PLoS Biol 3, e69.
  5. Hauf, S. et al. (2001) Science 293, 1320-3.
  6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
  7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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