Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Reptin/RuvBL2 (D8N3J) Rabbit mAb #12668

No. Size Price
12668S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12668 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 48 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Xenopus, Zebrafish, Bovine, Dog, Pig, Guinea Pig,

Specificity / Sensitivity

Reptin/RuvBL2 (D8N3J) Rabbit mAb recognizes endogenous levels of total Reptin/RuvBL2 protein.

Reptin/RuvBL2 (D8N3J) Rabbit mAb 兔单抗能够检测内源性Reptin/RuvBL2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly263 of human Reptin/RuvBL2 protein.

该单克隆抗体是采用合成的与人源Reptin/RuvBL2蛋白的Gly263残基周围序列相对应的肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Reptin/RuvBL2 (D8N3J) Rabbit mAb. Western blot方法检测不同细胞系的提取物,使用的抗体为Reptin/RuvBL2 (D8N3J) Rabbit mAb。

Background

Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7).Reptin plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters, where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex. Reptin is involved in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10).

Reptin/RuvBL2和Pontin/RuvBL1是与ATPase密切相关的成员,而ATPase是与多样的细胞内活动(AAA+)蛋白质的超家族有关,这些蛋白质显示了与细菌RuvB蛋白的同源性,RuvB蛋白可促进霍利迪连结体的分支迁移法(1)。两个蛋白能够一起被发现作为染色体重塑和修饰复合物的一部分,例如INO80、TIP60、SRCAP和Uri1,这些复合物都涉及到转录调控(1,2)。这些复合物中的成员Reptin涉及到由c-Myc、β-catenin和E1A驱动的致癌的转化,而这些作为一个本质的靶基因转录辅助调节因子(2-7)。Reptin也在调节细胞内缺氧反应中起着重要作用。低氧可诱导Reptin甲基化,这是通过甲基转移酶G9a导致它招募到缺氧反应启动子上,在那里它能负性调节这些基因的转录(8)。除了转录调控角色之外,Reptin也参与端粒酶生物合成过程,因为是端粒酶复合物的一部分,并且它参与DNA损伤反应,因为是TIP60乙酰转移酶复合物的一部分,该复合物可刺激ATM激酶活性,这对于涉及检测点激活和DNA修复蛋白质的磷酸化是必须的(9,10)。

  1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
  2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
  3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
  4. Kim, J.H. et al. (2005) Nature 434, 921-6.
  5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
  6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
  7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
  8. Lee, J.S. et al. (2010) Mol Cell 39, 71-85.
  9. Venteicher, A.S. et al. (2008) Cell 132, 945-57.
  10. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.

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