Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 (D3Z2G) Rabbit mAb #12640

sc-482   sc-8019  

No. Size Price
12640S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12640 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 79, 86 Rabbit IgG
IP 1:100
F 1:200
IF-IC 1:1000
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Bovine, Pig,

Specificity / Sensitivity

Stat3 (D3Z2G) Rabbit mAb recognizes endogenous levels of total Stat3 protein.

Stat3 (D3Z2G) Rabbit mAb兔单抗能够检测内源性Stat3总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly700 of human Stat3 protein.




Confocal immunofluorescent analysis of serum-starved HeLa cells, untreated (upper left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30 min; upper right), and serum-starved PC-3 cells, untreated (lower right) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30 min; lower right), using Stat3 (D3Z2G) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).共聚焦分析血清饥饿的HeLa细胞,未处理(左上图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30分钟; 右上图),以及血清饥饿的PC-3细胞,未处理(左下图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30分钟;右下图),使用抗体是Stat3 (D3Z2G) Rabbit mAb (绿色) 和 β-Actin (8H10D10) Mouse mAb #3700 (红色)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of of PC-3 (blue) and HeLa (green) cells using Stat3 (D3Z2G) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术分析PC-3(蓝色)和HeLa细胞(绿色),使用抗体是Stat3 (D3Z2G) Rabbit mAb。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412用来作为二抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 (D3Z2G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HepG2细胞中的交联染色质,细胞通过饥饿过夜或者使用Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 分钟) 或10 μl 的 Stat3α (D1A5) XP® Rabbit mAb或2 μl 的 Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663,和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stat3 (D3Z2G) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). PC-3 cells are negative for Stat3. Western blot分析多种细胞系的细胞提取物,使用抗体是Stat3 (D3Z2G) Rabbit mAb (上图), 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 PC-3细胞为Stat3阴性。



Immunoprecipitation of Stat3 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Stat3 (D3Z2G) Rabbit mAb (lane 3). Lane 1 is 10% input. 对HeLa细胞提取物中的Stat3进行免疫沉淀,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Stat3 (D3Z2G) Rabbit mAb (泳道3)。泳道1为10%的加入量。


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路中重要的信号分子(1),并且对于小鼠胎儿发育是必须的(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性肿瘤的可能(5) ,而且也具有抗凋亡活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核后与DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化的调控(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型、配体方向或者细胞发育的成熟阶段(10)。值得注意的是,Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

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