Cell Signaling Technology

Product Pathways - NF-kB Signaling

Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb #12629

No. Size Price
12629S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
12629 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Transfected Only 65 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb recognizes overexpressed levels of NF-κB p65 protein only when acetylated at Lys310.

Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb兔单抗仅仅能够检测过表达NF-κB p65的Lys310位点乙酰化。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys310 of human NF-κB p65 protein.

单克隆抗体是通过合成肽免疫动物制备,这种合成肽是人源 NF-κB p65 蛋白Lys310周围的肽段。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged human NF-κB p65 (hNF-κB p65; +) and HA-tagged human p300 (hp300-HA; +), using Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower).Western blot分析293T细胞的细胞提取物,对照转染(-)或转染表达Myc/DDK标签的人源NF-κB p65 (hNF-κB p65; +)的构建质粒以及HA标签的人源p300 (hp300-HA; +)构建质粒;使用抗体是Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (上图) 或 NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (下图)。



Immunoprecipitation of acetyl-NF-κB p65 (Lys310) from 293T cells, cotransfected with Myc/DDK-tagged human NF-κB p65 and HA-tagged human p300, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb.对293T细胞中的acetyl-NF-κB p65 (Lys310)进行免疫共沉淀,共转染Myc/DDK标签的人源NF-κB p65 和HA标签的人源p300,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或 Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb。


Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

核因子κ B(NF-κB)/Rel 家族的转录调控因子在炎症反应和免疫反应中发挥了至关重要的作用(1,2)。在哺乳动物中一共有5个家族成员: RelA, c-Rel, RelB, NF-κB1 (p105/p50) 和 NF-κB2 (p100/p52)。 p105 和 p100 在蛋白水解酶的作用下分别形成p50 和 p52。Rel与p50和p52形成二聚体,此复合体能够结合到DNA上调控转录。在未刺激的状态下, NF-κB 在IκB抑制剂作用下在细胞质中处于非活性状态(3-5)。 NF-κB激活因子能够诱导 IκB 蛋白的磷酸化, 这就使IκB能够快速的经过泛素化-蛋白酶通路降解从而释放 NF-κB,激活的NF-κB入核调控基因的表达(6-8)。 NIK 和 IKKα (IKK1) 调节磷酸化并促使NF-κB2 (p100) 生成p52, p52之后会入核发挥功能(9-11)。

NF-κB assembly with IκB, DNA binding, and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221, and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-κB (12-14).

NF-κB 与IκB的结合、与DNA的结合和转录活性, 会受到p300/CBP乙酰基转移酶调控,其修饰位点为Lys218, Lys221 和 Lys310 (12-14)。此过程受到组蛋白乙酰基酶(HDACs)反向调控,几个HDAC抑制因子被发现能够激活NF-κB (12-14)。

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