Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CTR9 (D1Z4F) Rabbit mAb #12619

No. Size Price
12619S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12619 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 140 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig,

Specificity / Sensitivity

CTR9 (D1Z4F) Rabbit mAb recognizes endogenous levels of total CTR9 protein.

CTR9 (D1Z4F) Rabbit mAb 兔单抗能够检测内源性CTR9总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CTR9 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CTR9 (D1Z4F) Rabbit mAb.Western blot方法检测不同细胞系的提取物,使用的抗体为 CTR9 (D1Z4F) Rabbit mAb。


The PAF (RNA polymerase II (RNAPII) associated factor) complex was initially identified in yeast and is comprised of subunits PAF1, Leo1, Ctr9, Cdc73, RTF1 and Ski8 (1,2). The PAF complex plays an important role in transcription initiation and elongation by RNAPII by regulating the establishment of proper histone modifications such as histone H2B ubiquitination and the recruitment of the histone chaperone FACT (facilitates chromatin transcription) (3-5). The PAF complex also plays a role in mRNA processing and maturation by interacting with, and recruiting, the cleavage and polyadenylation specificity factor and cleavage stimulation factor complexes via the Cdc73 subunit. (6,7). In addition, the Ski8 subunit of the PAF complex is part of the hSKi complex, which regulates RNA surveillance, suggesting an important function of the complex in coordinating events associated with proper RNA maturation during transcription (1,8).

PAF(RNA polymerase II (RNAPII) associated factor)复合物最初是在酵母中被鉴定出来的,由PAF1, Leo1, Ctr9, Cdc73, RTF1 和Ski8 (1,2)组成(1,2)。PAF复合物是RNAPII街道的转录起始和延伸过程中一个非常重要的角色,RNAPII可调控如组蛋白H2B泛素化等正确的组蛋白修饰的确立,还可以调控组蛋白伴侣FACT( facilitates chromatin transcription)的募集(3-5)。同时,PAF复合物还在mRNA加工和成熟过程中扮演重要的角色,因为它可以通过Cdc73亚基与剪切和多聚腺苷酸化特异性因子以及剪切刺激因子复合物相互作用,并募集他们(6,7)。另外PAF复合物的Ski8亚基还是hSKi复合物的一部分,而hSKi复合物能够调控RNA监控,这意味着PAF复合物在转录期间与与正确的RNA成熟有关的协调事件中发挥重要功能(1,8)。

  1. Jaehning, J.A. (2010) Biochim Biophys Acta 1799, 379-88.
  2. Chaudhary, K. et al. (2007) Oncogene 26, 7499-507.
  3. Pavri, R. et al. (2006) Cell 125, 703-17.
  4. Rozenblatt-Rosen, O. et al. (2005) Mol Cell Biol 25, 612-20.
  5. Zhu, B. et al. (2005) Genes Dev 19, 1668-73.
  6. Rozenblatt-Rosen, O. et al. (2009) Proc Natl Acad Sci U S A 106, 755-60.
  7. Farber, L.J. et al. (2010) Mol Carcinog 49, 215-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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