Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HEXIM1 (D5Y5K) Rabbit mAb #12604

No. Size Price
12604S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12604 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 60 Rabbit IgG
IP 1:100
F 1:1600
IF-IC 1:1200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

HEXIM1 (D5Y5K) Rabbit mAb recognizes endogenous levels of total HEXIM1 protein.

HEXIM1 (D5Y5K) Rabbit mAb兔单抗能够检测内源性HEXIM1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.

该单克隆抗体是采用合成的与人源HEXIM1蛋白Gly63残基周围序列相对应的肽段免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测HeLa细胞,使用的抗体为Di- HEXIM1 (D5Y5K) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HEXIM1 (D5Y5K) Rabbit mAb.Western blot方法检测不同细胞系的提取物,使用的抗体为HEXIM1 (D5Y5K) Rabbit mAb。

IP

IP

Immunoprecipitation of HEXIM1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HEXIM1 (D5Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HEXIM1 (D5Y5K) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀HEXIM1,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 HEXIM1 (D5Y5K) Rabbit mAb (lane 3)。Lane 1为10% input。 使用HEXIM1 (D5Y5K) Rabbit mAb进行Western blot检测。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with JQ1 (500nM, 72 hours; green), using HEXIM1 (D5Y5K) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.

Background

Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).

Hexamethylene bisacetamide-induced protein 1 (HEXIM1)最初是在血管平滑肌中被鉴定出来的,该蛋白经分化试剂六亚甲基二乙酰胺处理后可被上调(1)。HEXIM1能够结合到一个高丰度的非编码RNA 7SK RNA,一起作为positive transcription elongation b (P-TEFb)的强力抑制剂(2,3)。P-TEFb可使RNA polymerase II最大亚基的羧基端结构域磷酸化,并且是转录延伸的一个重要调控蛋白(4-8)。7SK RNA结合的HEXIM1可与P-TEFb蛋白的Cyclin T1 亚单位相互作用,这隔绝它在一个非活性形式,从而导致转录抑制(2,3)。HEXIM1/7SK可调控细胞中 RNAP-TEFb蛋白非活性与活性形式的相对比例,这种调控作用被认为在许多细胞内基因表达进程调控中有至关重要的作用,例如雌激素和糖皮质激素受体调控的基因(9-12)。

  1. Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
  2. Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
  3. Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
  4. Buratowski, S. (2009) Mol Cell 36, 541-6.
  5. Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
  6. Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
  7. Wada, T. et al. (1998) EMBO J 17, 7395-403.
  8. Yamada, T. et al. (2006) Mol Cell 21, 227-37.
  9. Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
  10. Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
  11. Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
  12. Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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