Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) #12602

No. Size Price
12602S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
12602 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) recognizes human caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody detects cleavage products containing the pro-domain with the p18 subunit, as well as the p18 subunit alone.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human caspase-8 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (green), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate).

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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