Product Pathways - Apoptosis
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) #12602
|12602S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|12602||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) recognizes human caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody detects cleavage products containing the pro-domain with the p18 subunit, as well as the p18 subunit alone.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human caspase-8 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496.
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-27.
- Boldin, M.P. et al. (1996) Cell 85, 803-15.
- Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9496 Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb
- 9978 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (PE Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条