Cell Signaling Technology

Product Pathways - Transcription Factors

MITF (D5G7V) Rabbit mAb #12590

Microphthalmia   sc-10999  

No. Size Price
12590S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12590 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 50-75 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Specificity / Sensitivity

MITF (D5G7V) Rabbit mAb recognizes endogenous levels of total MITF protein. The antibody is predicted to recognize all known isoforms.

MITF (D5G7V) Rabbit mAb兔单抗可以识别内源性的总MITF蛋白。预测该抗体可以识别所有的MITF亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly335 of human MITF protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MITF (D5G7V) Rabbit mAb.

使用MITF (D5G7V) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 SK-MEL-28 cells and either 10 μl of MITF (D5G7V) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Tyrosinase Promoter Primers #12970, human VMD2 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 SK-MEL-28细胞与10μl的MITF (D5G7V) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human Tyrosinase Promoter 引物 #12970,human VMD2 intron 1 引物,和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相比input染色质总量进行相对定量,input染色质量设定值为1。


Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression of cell and tissue types (6).

眼球相关转录因子(MITF)是一个碱性螺旋-环-螺旋锌指转录因子,最广为人知是它在黑色素细胞,眼科和破骨细胞发展中的作用(1-3)。人体中MITF可以在黑色素瘤中发挥作用(4),MITF基因突变与2型Waardenburg 综合症,一种听觉-色素综合症表现为神经嵴源性干细胞发育缺陷(5)。MITF发现了至少12种亚型,表现出不同的细胞和组织特异性(6)。

  1. Hershey, C.L. and Fisher, D.E. (2004) Bone 34, 689-96.
  2. Nomura, S. et al. (2001) J Bone Miner Metab 19, 183-7.
  3. Widlund, H.R. and Fisher, D.E. (2003) Oncogene 22, 3035-41.
  4. Levy, C. et al. (2006) Trends Mol Med 12, 406-14.
  5. Read, A.P. and Newton, V.E. (1997) J Med Genet 34, 656-65.
  6. Tachibana, M. (2000) Pigment Cell Res 13, 230-40.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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