Cell Signaling Technology

Product Pathways - Transcription Factors

CXXC1 Antibody #12585

CpG binding protein  

No. Size Price
12585S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12585 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 82 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CXXC1 Antibody recognizes endogenous levels of total CXXC1 protein.

CXXC1 Antibody 能够检测内源性CXXC1的总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of human CXXC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人源CXXC1 蛋白的Asp117残基周围序列相对应的肽段免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CXXC1 Antibody. Western blot方法检测不同细胞系的提取物,使用的抗体为CXXC1 Antibody。

Background

The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine Cxxc1 gene results in early embryonic lethality while Cxxc1-null embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation in vitro following withdrawal of leukemia inhibitory factor LIF (6).

CXXC finger protein 1 (CXXC1, CGBP, CFP1) 是人SET1 组蛋白甲基转移酶复合体的一个关键业绩(1,2),该复合体能够甲基化组蛋白H3的Lys4位点从而产生一个转录激活启动子的标志(3,4)。CXXC1在CpG 岛含量丰富,在这里其可以选择性的与非甲基化 CpG motif 结合从而在global H3K4 甲基化与CpG岛建立连接(5)。研究揭示了CXXC1的一个功能,就是通过与DNMT1的直接相互作用从而维持胞嘧啶甲基化(6-9)。CXXC1表观遗传功能对于正常的胚胎发育是至关重要的。定点敲除小鼠的Cxxc1基因会导致早期胚胎致死性,而无Cxxc1基因的胚胎干细胞会呈现出更快的凋亡,并且在去除leukemia inhibitory factor LIF后无法在体外进行分化(6)。

  1. Xu, C. et al. (2011) Nat Commun 2, 227.
  2. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
  3. Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
  4. Clouaire, T. et al. (2012) Genes Dev 26, 1714-28.
  5. Thomson, J.P. et al. (2010) Nature 464, 1082-6.
  6. Carlone, D.L. et al. (2005) Mol Cell Biol 25, 4881-91.
  7. Tate, C.M. et al. (2009) Mol Cell Biol 29, 3817-31.
  8. Young, S.R. et al. (2006) J Biol Chem 281, 37034-44.
  9. Butler, J.S. et al. (2008) DNA Cell Biol 27, 533-43.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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