Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human His6IGFBP3 (hHis6IGFBP3) #12579

No. Size Price
12579LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
12579LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
12579SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
12579SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
12579 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant Human His6IGFBP3 (hHis6IGFBP3) Gly28-Lys291 (Accession #NP_17936) was expressed in human 293 cells at Cell Signaling Technology.

在人293细胞中表达重组人His6 IGFBP3 (hHis6IGFBP3) Gly28-Lys291 (Accession #NP_17936)。

Molecular Characterization

Recombinant N-terminally His6-tagged hIGFBP3 has a calculated MW of 30,7123 Da. DTT reduced and nonreduced protein migrate as 50 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant hHis6IGFBP3 was verified by amino acid sequencing.

重组N端His标签的 hIGFBP3分子量30,7123 Da。DTT还原和非还原蛋白迁移大小为50 kDa。SDS-PAGE中低迁移率和均质性是由糖基化引起的。预测的重组hHis6IGFBP3的氨基端序列通过氨基酸测序确认。

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hHisIGFBP3. All lots are greater than 95% pure.

通过SDS-PAGE确认6 μg还原(+)和未还原(-)重组hHisIGFBP3纯度大于>95%。所有的批次纯度都大于>95%。

Bioactivity

The bioactivity of hHis6IGFBP3 was determined by inhibition of IGF-I induced AKT phosphorylation in human dermal fibroblasts. The ED50 of each lot is between 2.5-9 ng/ml.

hHis6IGFBP3的活性通过抑制人皮肤成纤维细胞中IGF- I诱导的AKT磷酸化而测定。

Bioactivity

Bioactivity

The inhibition of IGF-I induced Akt phosphorylation by hHis6IGFBP3. Human dermal fibroblasts were treated with Human Insulin-like Growth Factor I (hIGF-I) # 8917 in the presence or absence of increasing concentrations of hHis6IGFBP3 for 10 minutes, lysed, and Akt1 (Ser473) phosphorylation was quantified using the PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160.

抑制IGF-I可以促进Akt被hHis6IGFBP3磷酸化。人皮肤成纤维细胞使用人胰岛素样生长因子I (hIGF-I) # 8917使用浓度逐渐增加的hHis6IGFBP3处理10分钟或不经过处理,然后裂解,使用PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160测定Akt1 (Ser473)磷酸化水平。

Coomassie Gel

Coomassie Gel

The purity of recombinant hHis6IGFBP3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant.

SDS-PAGE测定6 µg还原(+)或不还原(-)的重组hHis6IGFBP3的纯度。

Applications

Optimal concentration for the desired application should be determined by the user.

最佳使用浓度需要客户自行优化。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis6IGFBP3.

1 μg hHis6IGFBP3中内毒素低于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hHis6IGFBP3 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hHis6IGFBP3. Carrier free: Lyophilized from a 0.22 μm filtered solution of hHis6IGFBP3in 20 mM Tris, pH 7.2.

带载体:储存在含有 20 μg BSA 的20 mM Tris, pH 7.2 溶液中的1 μg hHHis6IGFBP3经过0.22 μm过滤后冻干。无载体:hHis6 IGFBP3 i在 20 mM Tris, pH 7.2经过0.22 μm过滤后冻干。

Background

IGFBP3 is a multifunctional protein that plays a key role in regulation of IGFI/II activity, cell proliferation, and death. One of six high-affinity IGF binding proteins, IGFBP3 is the major species in circulation and is bound in a complex with ALS to 99% of hepatic IGF-I (1). Proteolytic degradation of IGFBP3 increases the bioavailability and activity of the IGF I/II (1). However, some biological activities of IGFBP3 are independent of the IGF/IGF-IR axis. IGFBP3 potentiates EGF-induced breast cancer cell proliferation in vitro by enhancing ERK phosphorylation and sphingosine kinase-mediated EGFR trans-activation (2,3). Conversely, IGFBP3 has been shown to induce apoptosis and inhibit NF-κB activity (4).

IGFBP3是一种多功能的蛋白,在调控IGFI/II活性,细胞增殖和细胞死亡上发挥关键作用。作为六个高亲和IGF结合蛋白之一,IGFBP3主要位于循环系统中,并和ALS结合成为复合物后与99%肝脏中的IGF- I结合 (1)。IGFBP3的蛋白降解过程增加了IGF I/II的生物活性(1)。但是,IGFBP3的某些生物性能是不依赖于IGF/IGF-IR信号通路的。体外实验表明IGFBP3通过增强ERK的磷酸化和sphingosine激酶介导的EGFR转化,从而增加可能引起了EGF诱发的乳腺癌(2,3)。相反的是,IGFBP3也被证明可以诱导凋亡并抑制NF-κB活性(4)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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