Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TBP (D5G7Y) Rabbit mAb #12578

No. Size Price
12578S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12578 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 38 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

TBP (D5G7Y) Rabbit mAb recognizes endogenous levels of total TBP protein.

TBP (D5G7Y) Rabbit mAb兔单抗能够检测内源性TBP总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of human TBP protein.

该单克隆抗体采用合成的与人源TBP蛋白Ala110残基周围序列相对应的肽段免疫动物而生产的。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of TBP (D5G7Y) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human γ-Actin Promoter Primers #5037, SimpleChIP® Human GAPDH Promoter Primers #4471 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 对4 x 106 HeLa细胞中交联过的染色质进行染色质免疫沉淀,所用抗体为10 µl Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb 或2 µl Normal Rabbit IgG #2729,富集到的DNA经过实时PCR定量,所使用产品为SimpleChIP® Human γ-Actin Promoter Primers #5037, SimpleChIP® Human GAPDH Promoter Primers #4471 以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TBP (D5G7Y) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为TBP (D5G7Y) Rabbit mAb。

IP

IP

Immunoprecipitation of TBP from HCT 116 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TBP (D5G7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TBP (D5G7Y) Rabbit mAb.从 HCT 116细胞提取物中免疫沉淀TBP,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或TBP (D5G7Y) Rabbit mAb (lane 3)。Lane 1为10% input。 使用TBP (D5G7Y) Rabbit mAb进行Western blot检测。

Background

TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex.

TATA-binding protein (TBP)是一个广泛表达的细胞核蛋白,该蛋白在一般转录因子蛋白复合物TFIID中心起作用(1-3)。TFIID包含TBP和13 TBP相关因子(TAFs),该蛋白有助于转录前起始复合物的形成,该复合物是由多种蛋白复合物 (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II)组装形成的,在转录的起始阶段这些蛋白质结合到一个基因启动子上(1-3)。一旦前起始复合物形成,RNA polymerase II有助于延伸和基因转录。TBP通过结合到TATA-box序列从而发挥TFIID的招募功能,TATA-box序列发现在许多蛋白质编码基因的转录起始位点上游的大约25碱基对。此外,许多转录激活蛋白与TBP和多种TAF蛋白相互作用,从而有助于TFIID的招募和前起始复合物的形成。

  1. Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
  2. Berk, A.J. (2000) Cell 103, 5-8.
  3. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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