Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

RUNX2 (D1L7F) Rabbit mAb #12556

sc-101145   sc-10758  

No. Size Price
12556S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12556 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55-62 Rabbit IgG
IP 1:50
F 1:800
IF-IC 1:1600
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

RUNX2 (D1L7F) Rabbit mAb recognizes endogenous levels of total RUNX2 protein.

RUNX2 (D1L7F) Rabbit mAb可以识别内源性的总RUNX2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein.

此单克隆抗体由合成重组蛋白免疫动物产生,该重组蛋白与人RUNX2蛋白Ala273邻近氨基酸残基序列一致。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Saos-2 (left) and LNCaP (right) cells using RUNX2 (D1L7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

使用RUNX2 (D1L7F) Rabbit mAb (绿色)对Saos-2细胞 (左) 和LNCaP 细胞(右)进行共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin(红色)标记。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of LNCaP (blue) and Saos-2 (green) cells using RUNX2 (D1L7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

使用RUNX2 (D1L7F) Rabbit mAb对Saos-2 细胞(蓝色) 和LNCaP 细胞(绿色)进行流式分析。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 偶联) #441用作二抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from Saos-2 and LNCaP cells using RUNX2 (D1L7F) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.

使用RUNX2 (D1L7F) Rabbit mAb和β-Actin (D6A8) Rabbit mAb

兔单抗 #8457对Saos-2细胞和LNCaP细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Saos-2 cells and either 10 μl of RUNX2 (D1L7F) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human RUNX1 Intron 1 primers, SimpleChIP® Human RUNX2 Promoter Primers #12376, human FRA10AC1 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 Saos-2细胞与10μl的RUNX2 (D1L7F) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human RUNX1 Intron 1 引物和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量通过比对Input染色质总量进行相对定量,Input染色质量设定值为1。

IP

IP

Immunoprecipitation of RUNX2 from Saos-2 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RUNX2 (D1L7F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RUNX2 (D1L7F) Rabbit mAb.

使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 RUNX2 (D1L7F) Rabbit mAb (lane 3)对Saos-2细胞中的RUNX2蛋白进行免疫沉淀实验。Lane1是10%上样量。RUNX2 (D1L7F) Rabbit mAb用作western blot分析。

Background

Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers including prostate cancer and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10).

Runt-相关转录因子2(RUNX2)是RUNX转录因子家族的成员。它参与了成骨细胞分化和骨骼肌形成。RUNX2通过结合到增强子或启动子的中心位置调节多种基因转录,包括osteopontin,bone sialoprotein, 以及osteocalcin (1-3)。RUNX2对于成骨细胞的成熟以及膜内和软骨内固化都至关重要。RUXN2基因突变会与骨骼发育异常锁骨颅骨发育不全相关(4-6)。RUNX2也在多种人类癌症中异常表达包括前列腺癌和乳腺癌。它在前列腺和乳腺癌细胞的迁移,侵袭和骨转移中发挥了重要功能(7-10).

  1. Viereck, V. et al. (2002) J Cell Biochem 86, 348-56.
  2. Willis, D.M. et al. (2002) J Biol Chem 277, 37280-91.
  3. Tu, Q. et al. (2008) J Cell Physiol 217, 40-7.
  4. Quack, I. et al. (1999) Am J Hum Genet 65, 1268-78.
  5. Cardoso, B.M. et al. (2010) Clin Dysmorphol 19, 150-2.
  6. Han, M.S. et al. (2010) J Cell Biochem 110, 97-103.
  7. Akech, J. et al. (2010) Oncogene 29, 811-21.
  8. van der Deen, M. et al. (2010) J Cell Biochem 109, 828-37.
  9. Barnes, G.L. et al. (2003) Cancer Res 63, 2631-7.
  10. Barnes, G.L. et al. (2004) Cancer Res 64, 4506-13.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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