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12520
Vorinostat (SAHA)
激活剂与抑制剂
化学调节物

Vorinostat (SAHA) #12520

Citations (0)
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-Histone H3 (Lys14) Antibody #4318 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower).
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower).
Chemical structure of vorinostat (SAHA).

Product Usage Information

Vorinostat is supplied as a lyophilized powder. For a 20 mM stock, reconstitute the 5 mg in 945.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 2-24 hr. Soluble in DMSO at 66 mg/ml; soluble in ethanol at 2 mg/ml with slight warming; very poorly soluble in water with maximum solubility ~20-50 µM.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 264.32 g/mol
Purity >99%
Molecular Formula C14H20N2O3
CAS 149647-78-9
Solubility Soluble in DMSO at 66mg/ml and EtOH at 2mg/ml.

Background

Vorinostat (SAHA) is a histone deacetylase (HDAC) inhibitor that acts by binding to and blocking the active site of the enzyme (1). Both class I and class II HDACs are inhibited by vorinostat at nanomolar concentrations (1-3). Research studies have shown that vorinostat causes the accumulation of acetylated histones, certain transcription factors, and other nonhistone proteins, which regulate gene expression and protein function. This leads to cell cycle arrest, differentiation, and/or apoptosis in many transformed cell types treated with micromolar concentrations of vorinostat (1,4-7).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 12520S
Cat. # Size Price Inventory
12520S
5 mg