Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SirT6 (D8D12) Rabbit mAb #12486

de-acetylase   deacetylase   sirt   sirtuin  

No. Size Price
12486S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12486 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 42, 36 Rabbit IgG
IP 1:200
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SirT6 (D8D12) Rabbit mAb recognizes endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins.

SirT6(D8D12)Rabbit mAb兔单抗能够检测内源性的SirT6总蛋白水平。该抗体不会与其他sirtuin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length mouse SirT6 protein.

该单克隆抗体是由针对人SirT6蛋白全长的重组蛋白免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells, expressing either non-targeting shRNA (shNT) (left) or shSirT6 (right), using SirT6 (D8D12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, expressing either non-targeting shRNA (shNT) or shSirT6, using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT6 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SirT6 WT and KO MEF were kindly provided by Dr. David Lombard, University of Michigan.

IP

IP

Immunoprecipitation of SirT6 from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3) or SirT6 (D8D12) Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using SirT6 (D8D12) Rabbit mAb.

Background

The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2).

沉默信息调节因子(SIR2)基因家族是一类高度保守的基因,能够编码烟酰胺腺嘌呤二核苷酸(NAD)依赖性的去乙酰化酶,亦称III类组蛋白去乙酰化酶。在这些基因中,酿酒酵母Sir2是最早被发现,并且了解得最清楚的。Sir2 参与了交配型基因座的沉默,端粒维持,DNA损伤应答和细胞衰老(1)。SirT6,是Sir2在哺乳动物中的同源基因,是一种核仁、染色质相关蛋白,能够通过碱基修复(BER)信号通路以正常维持基因组的完整性(2-4)。 BER通路可以修复内源烷化、氧化和脱氨基作用中自发的单链DNA损伤。SirT6缺乏的小鼠,对DNA损伤药剂的敏感性明显增强,包括烷化药剂MMS和H2O2 (2)。另外,这些小鼠中还显示出基因组的不稳定性,其断裂染色体、分离的着丝粒以及缺口的出现频率明显增加(2)。SirT6可能通过对通路中DNA Polβ或其他核心组分的去乙酰化作用来调控BER通路(2)。

  1. Guarente, L. (1999) Nat Genet 23, 281-5.
  2. Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
  3. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
  4. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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