Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCA1 (D4Q7V) Rabbit mAb #12483

sc-367689  

No. Size Price
12483S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12483 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 130 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SMARCA1 (D4Q7V) Rabbit mAb recognizes endogenous levels of total SMARCA1 protein.

SMARCA1 (D4Q7V) Rabbit mAb兔单抗能够检测内源性的SMARCA1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro73 of human SMARCA1 protein.

该单克隆抗体是采用合成的与人源SMARCA1蛋白Pro73残基周围序列相对应的肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western Blot anaylsis of extracts from various cell lines using SMARCA1 (D4Q7V) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为SMARCA1 (D4Q7V) Rabbit mAb。

IP

IP

Immunoprecipitation of SMARCA1 from PANC-1 cell extracts using Rabbit (DA1E) IgG XP® Isotype Control #3900 (lane 2) or SMARCA1 (D4Q7V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SMARCA1 (D4Q7V) Rabbit mAb.从 PANC-1细胞提取物中免疫沉淀SMARCA1,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或SMARCA1 (D4Q7V) Rabbit mAb (lane 3)。Lane 1为10% input。 使用SMARCA1 (D4Q7V) Rabbit mAb进行Western blot检测。

Background

SMARCA1 (SNF2L) is one of the two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). The ISWI chromatin remodeling complexes were first identified in Drosophila and have been shown to remodel and alter nucleosome spacing in vitro (2). SMARCA1 is the catalytic subunit of the nucleosome remodeling factor (NURF) and CECR2-containing remodeling factor (CERF) complexes (3-5). The NURF complex plays an important role in neuronal physiology by promoting neurite outgrowth and regulation of Engrailed homeotic genes that are involved in neuronal development in the mid-hindbrain (3). NURF is also thought to be involved in the maturation of T cells from thymocytes by regulating chromatin structure and expression of genes important for T cell development (6). The largest subunit of the NURF complex, BPTF, is required for proper development of mesoderm, endoderm, and ectoderm tissue lineages, suggesting a role for SMARCA1 in the development of the germ layers in mouse embryo (7). Disruption of the CERF complex by deletion of CECR2, an interacting partner of SMARCA1, is associated with the neural tube defect exencephaly, linking the CERF complex with regulation of neurulation (4).

SMARCA1 (SNF2L)是ISWI (imitation switch) ATPases的两个直系同源物之一,由哺乳动物基因编码(1)。 ISWI染色质重塑复合物首先在果蝇中被鉴定出来,并且在体外去改造和改变核小体间隔(2)。SMARCA1是NURF(nucleosome remodeling factor)和CERF(CECR2-containing remodeling factor) 复合物的催化亚基(3-5)。NURF复合物在神经生理学中具有重要作用,它能够促进神经突起生长并调节参与中后脑中神经元发育的Engrailed同源异型基因 (3)。NURF被认为是通过对染色质结构和T细胞发育中重要基因表达的调控从而参与到胸腺来源的T细胞成熟过程中的 (6)。NURF复合物的最大亚基BPTF是中胚层、内胚层和外胚层组织谱系所需要的,这意味着SMARCA1在小鼠胚胎的胚层发育中起作用 (7)。CECR2是一个SMARCA1相互作用的蛋白质,由CECR2缺失引起的CERF复合物的分解会导致与露脑性神经管缺陷,从而将CERF与神经胚形成联系在一起(4)。

  1. Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem 77, 1145-56.
  2. Erdel, F. and Rippe, K. (2011) FEBS J 278, 3608-18.
  3. Barak, O. et al. (2003) EMBO J 22, 6089-100.
  4. Banting, G.S. et al. (2005) Hum Mol Genet 14, 513-24.
  5. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  6. Landry, J.W. et al. (2011) Genes Dev 25, 275-86.
  7. Landry, J. et al. (2008) PLoS Genet 4, e1000241.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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