Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCAD1 Antibody #12458

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Pig,

Specificity / Sensitivity

SMARCAD1 Antibody recognizes endogenous levels of total SMARCAD1 protein. This antibody also cross-reacts with a protein of unknown origin at 40 kDa.

SMARCAD1 Antibody能够检测内源性SMARCAD1总蛋白水平。该抗体还能与一个未知来源的40kDa蛋白产生相互作用。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMARCAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体经由人工合成的人源SMARCAD1蛋白近氨基末端的多肽片段免疫动物而生产的。通过蛋白A和多肽的亲和层析方式纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMARCAD1 Antibody.Western blot方法检测多种细胞系的提取物,使用的抗体为 SMARCAD1 Antibody。

Background

SMARCAD1 is a SWI/SNF-like chromatin remodeling protein that plays a critical role in the maintenance of heterochromatin domains following DNA replication and proper chromosome segregation during mitosis (1-3). SMARCAD1 can be found in association with transcription repressor KAP1, histone deacetylases HDAC1/2, and lysine methyltransferase G9a/GLP, which are recruited to sites of DNA replication by PCNA. These proteins facilitate deacetylation of histones and methylation of histone H3 Lys9 to re-establish heterochromatin domains, such as centromeric regions (1). SMARCAD1 also plays a role in double stranded DNA break repair by facilitating DNA end resection and the subsequent repair by homologous recombination (4). Loss of SMARCAD1 results in increased sensitivity to DNA damaging agents, suggesting a role in the maintenance of genome stability.

SMARCAD1是一个SWI/SNF样染色质重塑蛋白,对于DNA复制之后异染色质结构域的维持以及有丝分裂期间染色体的正确分离有至关重要的作用(1-3)。经研究发现SMARCAD1与转录阻遏子 KAP1,组蛋白去乙酰化酶HDAC1/2以及赖氨酸甲基转移酶G9a/GLP联系在一起,这些都会被PCNA募集到DNA复制位点上。这些蛋白能够有助于组蛋白的去乙酰化以及组蛋白H3 Lys9位点的甲基化从而建立一个异染色质结构域,如着丝粒区(1)。SMARCAD1还可以促进DNA末端切除和随后的同源重组的修复,对于双链DNA断裂修饰是很重要(4)。SMARCAD1确实会导致提高对DNA损伤事件的敏感度,这意味着其在基因组稳定性维持的重要作用。

  1. Rowbotham, S.P. et al. (2011) Mol Cell 42, 285-96.
  2. Mermoud, J.E. et al. (2011) Cell Cycle 10, 4017-25.
  3. Jasencakova, Z. and Groth, A. (2011) Mol Cell 42, 267-9.
  4. Costelloe, T. et al. (2012) Nature 489, 581-4.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications