Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MacroH2A1.1 (D5F6N) Rabbit mAb #12455

No. Size Price
12455S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12455 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 40 Rabbit IgG
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

MacroH2A1.1 (D5F6N) Rabbit mAb recognizes endogenous levels of total MacroH2A1.1 protein. This antibody does not cross-react with other MacroH2A proteins, including MacroH2A1.2 and MacroH2A2.

MacroH2A1.1 (D5F6N) Rabbit mAb兔单抗能够识别内源性MacroH2A1.1总蛋白水平。该抗体不会与其他MacroH2A蛋白包括MacroH2A1.2和MacroH2A2发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human MacroH2A1.1 protein.

该单克隆抗体是采用合成的与人源MacroH2A1.1 蛋白Ala211残基周围序列相对应的肽段免疫动物而生产的。



Confocal immunofluorescent analysis of HeLa cells using MacroH2A1.1 (D5F6N) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

采用共聚焦免疫荧光术检测Hela细胞(左)和NIH/3T3细胞(右),使用的抗体为MacroH2A1.1 (D5F6N) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝使用DY-554 鬼笔环肽进行标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues using MacroH2A1.1 (D5F6N) Rabbit mAb.

Western blot方法检测不同细胞系的提取物,所用抗体为MacroH2A1.1 (D5F6N) Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MacroH2A1.1 (hMacroH2A1.1-DDK; +) or DDK-tagged full-length human MacroH2A1.2 (hMacroH2A1.2-DDK; +), using MacroH2A1.1 (D5F6N) Rabbit mAb (upper) and DYKDDDDK Tag (9A3) Mouse mAb #8146 (lower).

Western blot方法检测293细胞提取物,分别用模拟物(-)或构建的带有Myc/DDK标签的全长人MacroH2A1.1 (hMacroH2A1.1-Myc/DDK; +)、或带 DDK标签的全长人MacroH2A1.2 (hMacroH2A1.2-DDK; +),使用的抗体为 MacroH2A1.1 (D5F6N) Rabbit mAb 兔单抗(上图) 以及 DYKDDDDK Tag (9A3) Mouse mAb #8146 鼠单抗(下图)。


Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal “macro” domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to locally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8).

组蛋白macroH2A1 和 macroH2A2 由一类组蛋白H2A异构体家族组成。由于单个基因的剪接方式不同,MacroH2A1存在两种不同的异构体;在整个分化和发育过程中,macroH2A1.1的水平不断累积而macroH2A1.2的表达水平则保持不变(1)。MacroH2A1 和 macroH2A2由位于不同染色体上完全不同的两个基因编码(2,3)。这两个蛋白都含有一个氨基端组蛋白样区域,该区域64%的序列与规范的组蛋白H2A一致,同时还含有一个羧基端“macro”区域(1-3)。兼性异染色质中富含MacroH2A1 和 macroH2A2,包括雌性哺乳动物失活的X染色体以及衰老相关异染色质基因座(2-5)。MacroH2A1 和 macroH2A2都能通过抑制转录因子与染色质的结合来抑制基因转录,还能抑制p300对组蛋白的乙酰化作用以及SWI/SNF 和ACF的染色质重塑活性(6,7)。macroH2A1.1的Marco 结构域能够与ADP-核糖结合,并将macroH2A1.1募集到DNA损伤位点上以激活PARP,在该位点上它能够介导染色质重组从而局部调控DNA损伤应答(8)。而MacroH2A1.2 和 macroH2A2不能够结合多聚ADP-核糖也不能被募集到活化的PARP该位点上(8)。

  1. Pehrson, J.R. et al. (1997) J Cell Biochem 65, 107-13.
  2. Chadwick, B.P. and Willard, H.F. (2001) Hum Mol Genet 10, 1101-13.
  3. Costanzi, C. and Pehrson, J.R. (2001) J Biol Chem 276, 21776-84.
  4. Costanzi, C. and Pehrson, J.R. (1998) Nature 393, 599-601.
  5. Zhang, R. et al. (2005) Dev Cell 8, 19-30.
  6. Angelov, D. et al. (2003) Mol Cell 11, 1033-41.
  7. Doyen, C.M. et al. (2006) Mol Cell Biol 26, 1156-64.
  8. Timinszky, G. et al. (2009) Nat Struct Mol Biol 16, 923-9.

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