Cell Signaling Technology

Product Pathways - Neuroscience

CK1ε Antibody #12448

No. Size Price
12448S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12448 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 43 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CK1ε Antibody recognizes endogenous levels of total CK1ε protein. This antibody does not cross-react with CK1δ.

CK1ε Antibody兔多抗能够检测内源性的CK1ε总蛋白。该抗体不会与CK1δ产生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala349 of human CK1ε protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源CK1ε蛋白丙氨酸(349位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human CK1δ (CK1δ-Myc/DDK; +) or Myc-tagged full-length human CK1ε (CK1ε-Myc/DDK; +), using CK1ε Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western blot方法检测293T细胞提取物:空质粒转染(-)或带Myc-标签全长人CK1δ (CK1δ- Myc/DDK; +)或带Myc-标签全长人CK1ε (CK1ε-Myc/DDK; +)载体转染;使用的抗体是CK1εAntibody兔多抗(上图)或Myc-Tag (71D10) Rabbit mAb 兔单抗#2278(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CK1ε Antibody.

Western blot 检测多种细胞系提取物,使用的抗体是CK1ε Antibody兔多抗。

Background

Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).CK1ε is involved in many cellular processes such as differentiation (5-7), cell growth and apoptosis (8), and control of the circadian rhythm (9,10).

酪蛋白激酶I (CK1或CKI)是一个激酶家族的名字,这个家族蛋白由多个亚型组成(α, α', β, γ1-3, δ和ε),带有一个保守的N末端激酶结构域和一个可变的C末端序列,该序列决定了蛋白的亚细胞定位并调控酶的活性(1-3)。事实上,已确定在CKIε 的C末端存在着多抑制自磷酸化位点(3)。这种广泛表达的蛋白激酶家族已被发现参与了包括DNA修复,细胞形态学,Wnt信号在内的多个生物学过程(4)。可能被研究的最明确的CKI的作为是起始β-catenin45位丝氨酸磷酸化,产生GSK3底物基序(丝氨酸/苏氨酸-X-X-X-磷酸化丝氨酸)。CK1ε参与许多细胞过程,如分化(5-7),细胞生长和细胞凋亡(8)以及昼夜节律的控制(9,10)。

  1. Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
  2. Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
  3. Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
  4. Polakis, P. (2002) Curr. Biol. 12, R499-R501.
  5. Okamura, A. et al. (2004) Blood 103, 2997-3004.
  6. Swiatek, W. et al. (2006) J Biol Chem 281, 12233-41.
  7. Bischof, J. et al. (2011) PLoS One 6, e20857.
  8. Brockschmidt, C. et al. (2008) Gut 57, 799-806.
  9. Keesler, G.A. et al. (2000) Neuroreport 11, 951-5.
  10. Meng, Q.J. et al. (2008) Neuron 58, 78-88.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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