Cell Signaling Technology

Product Pathways - Autophagy Signaling

MTMR3 Antibody #12443

No. Size Price
12443S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12443 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 130-150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

MTMR3 Antibody recognizes endogenous levels of total MTMR3 protein. This antibody also cross-reacts with a protein of unknown origin at 48 kDa.

MTMR3 Antibody能识别内源性水平的总MTMR3蛋白。此抗体与48kDa处的来源不明的蛋白有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro600 of human MTMR3 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTMR3 Antibody.

Western blot 分析不同细胞系的提取物,使用的抗体是MTMR3 Antibody。


Myotubularin-related proteins are a family of phosphatases with emerging roles in cellular signaling and membrane trafficking (1,2). MTMR3 (Myotubularin-related protein 3), also known as FYVE-DSP1, contains an amino terminal pleckstrin homology (PH) domain and a carboxyl terminal FYVE domain. MTMR3 was first reported as a dual-specific phosphatase, having phosphatase activity toward phosphorylated serine, threonine, and tyrosine residues (3). Subsequent research studies reported that MTMR3 has phosphatase activity toward phosphoinositides, including phosphatadylinositol-3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) (4). Accumulation of PI3P by the class III phosphoinositide 3-kinase Vps34 is a key element in autophagosome formation (5). Inhibition of PI3P by MTMR3 can play an important role in suppressing autophagsome formation (6).

肌管素相关蛋白是在细胞信号和膜运输中新出现的一个磷酸酶类家族(1,2)。MTMR3(肌管素相关蛋白3),也称为FYVE-DSP1,包含一个氨基末端PH结构域和羧基末端FYVE结构域。MTMR3作为双特异性磷酸酶,是首次报道,它对磷酸化的丝氨酸、苏氨酸和酪氨酸残基有磷酸酶活性(3)。后续研究表明,MTMR3对磷酸肌醇有磷酸酶活性,包括磷酸肌醇-3-磷酸(PI3P)和3,5-二磷酸磷脂酰肌醇(PI(3,5)P2) (4)。由III级磷酸肌醇3-激酶Vps34引起的PI3P的积累,在自噬体形成过程中起到了关键作用(5)。由MTMR3引起的PI3P抑制,在抑制自噬体的形成中有重要作用(6)。

  1. Robinson, F.L. and Dixon, J.E. (2006) Trends Cell Biol 16, 403-12.
  2. Laporte, J. et al. (2002) Biochem Biophys Res Commun 291, 305-12.
  3. Zhao, R. et al. (2000) Biochem Biophys Res Commun 270, 222-9.
  4. Walker, D.M. et al. (2001) Curr Biol 11, 1600-5.
  5. Simonsen, A. and Tooze, S.A. (2009) J Cell Biol 186, 773-82.
  6. Taguchi-Atarashi, N. et al. (2010) Traffic 11, 468-78.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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