Cell Signaling Technology

Product Pathways - Autophagy Signaling

FIP200 (D10D11) Rabbit mAb #12436

No. Size Price
12436S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12436 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 200 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

FIP200 (D10D11) Rabbit mAb recognizes endogenous levels of total FIP200 protein.

FIP200 (D10D11) Rabbit mAb 兔单抗能识别内源性的FIP200总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FIP200 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FIP200 (D10D11) Rabbit mAb.

Western blot 分析不同细胞系的提取物,使用的抗体是 FIP200 (D10D11) Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 cells, untreated (-) or chloroquine-treated (50 μM, overnight; +) using FIP200 (D10D11) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Western blot 分析A172 细胞的提取物,未处理(-)或氯喹处理(50 μM, 过夜; +),使用的抗体是:FIP200 (D10D11) Rabbit mAb 兔单抗(上图),或者 α-Actinin (D6F6) XP® Rabbit mAb 兔单抗#6487 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expresssing full-length human FIP200 (hFIP200; +), using FIP200 (D10D11) Rabbit mAb.

Western blot分析 293T 细胞的提取物,对照转染(-)或全长的人 FIP200(hFIP200; +)载体转染,使用的抗体是FIP200 (D10D11) Rabbit mAb 兔单抗。



Immunoprecipitation of FIP200 from JJN-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FIP200 (D10D11) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FIP200 (D10D11) Rabbit mAb.

免疫沉淀分析JJN-3 细胞中提取的FIP200,使用的抗体是:Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(泳道2),FIP200 (D10D11) Rabbit mAb(泳道3)。泳道1是10% input。Western blot分析使用的抗体是FIP200 (D10D11) Rabbit mAb 兔单抗。


FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members (1). FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation (2). FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration (reviewed in 3). FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation (4). FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy (5-7).

FIP200(FAK家族激酶互作蛋白,200 kDa)与酪氨酸激酶Pyk2是在双杂交筛选时被发现的,并且 FIP200 能够抑制Pyk2激酶及其相关家族成员的活性(1)。随后,又在多重耐药筛选中发现FIP200,并被命名为RB1CC1,这是因为它能够诱导细胞毒性应激和调节RB1表达(2)。FIP200的功能与细胞凋亡、细胞周期进程、细胞生长和迁移相联系(回顾3)。最近又发现,FIP200能与ULK1相互作用,并且是自噬体形成的重要部分(4)。FIP200 与受mTOR调节的Atg13都是ULK1复合物的一部分,也是饥饿诱导的自噬所必需的(5-7)。

  1. Ueda, H. et al. (2000) J Cell Biol 149, 423-30.
  2. Chano, T. et al. (2002) Oncogene 21, 1295-8.
  3. Gan, B. and Guan, J.L. (2008) Cell Signal 20, 787-94.
  4. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
  5. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
  6. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
  7. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

Application References

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