Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Src (Ser17) (D7F2Q) Rabbit mAb #12432

No. Size Price
12432S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
12432 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 60 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Src (Ser17) (D7F2Q) Rabbit mAb recognizes endogenous levels of Src protein only when phosphorylated at Ser17.

Phospho-Src (Ser17) (D7F2Q)Rabbit mAb兔单抗能够检测内源性的Ser17磷酸化的Src蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser17 of human Src protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Src (Ser17) (D7F2Q) Rabbit mAb (upper) or Src (32G6) Rabbit mAb #2123 (lower).

未处理(-)或使用λ磷酸酶和牛小肠碱性磷酸酶(CIP)处理(+)的293T细胞抽取物,使用Phospho-Src (Ser17) (D7F2Q) Rabbit mAb兔单抗(上)或Src (32G6)Rabbit mAb兔单抗#2123(下)进行western blot分析。



Immunoprecipitation of phospho-Src (Ser17) from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Src (Ser17) (D7F2Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Src (Ser17) (D7F2Q) Rabbit mAb. A light-chain specific antibody was used as a secondary antibody.

使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(lane2)或Phospho-Src (Ser17) (D7F2Q)Rabbit mAb兔单抗(lane3)对293T细胞提取物的phospho-Src (Ser17)进行免疫沉淀实验。Lane1是10% input。Phospho-Src (Ser17) (D7F2Q)Rabbit mAb兔单抗用作western blot分析。一个轻链特异性抗体用作二抗。


The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的,但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化,能够上调酶活性;而蛋白羧基端尾部Tyr527位点被Csk磷酸化后,则会使酶的活性降低(2)。

Protein kinase A (PKA)-dependent phosphorylation of Src at Ser17 is thought to influence multiple signaling networks (3-5). This site has also been identified in a phospho-proteomic screen for substrates of mTOR (6).

蛋白激酶A(PKA)依赖的Src的Ser17磷酸化被认为会影响多条信号通路(3-5)。 通过磷酸化蛋白组学的筛选,该位点已被鉴定为mTOR的底物。(6)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94.
  4. Abrahamsen, H. et al. (2003) J Biol Chem 278, 17170-7.
  5. Obara, Y. et al. (2004) J Cell Sci 117, 6085-94.
  6. Hsu, P.P. et al. (2011) Science 332, 1317-22.

Application References

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