Cell Signaling Technology

Product Pathways - NF-kB Signaling

CARD9 Antibody #12416

No. Size Price
12416S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12416 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 62 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CARD9 Antibody recognizes endogenous levels of total CARD9 protein.

CARD9 抗体检测内源性CARD9总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CARD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1, U-937, and MUTZ-3 cells using CARD9 Antibody. Western blot分析THP-1、U-937和 MUTZ-3细胞的细胞提取物,使用抗体是using CARD9 Antibody。


CARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8).


  1. Bertin, J. et al. (2000) J Biol Chem 275, 41082-6.
  2. Hsu, Y.M. et al. (2007) Nat Immunol 8, 198-205.
  3. Gross, O. et al. (2006) Nature 442, 651-6.
  4. Bi, L. et al. (2010) J Biol Chem 285, 25969-77.
  5. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
  6. Strasser, D. et al. (2012) Immunity 36, 32-42.
  7. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
  8. Wu, W. et al. (2009) Nat Immunol 10, 1208-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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