Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate) #12413

No. Size Price
12413S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价 防伪查询
12413 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 100 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Zebrafish,

Specificity / Sensitivity

α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total α-actinin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe316 of human α-actinin protein.


This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Actinin (D6F6) XP® Rabbit mAb #6487.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate).


α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

  1. Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11.
  2. Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66.
  3. Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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